Structure and dynamics of a primordial catalytic fold generated by in vitro evolution

Fa An Chao, Aleardo Morelli, John C.Haugner Iii, Lewis Churchfield, Leonardo N. Hagmann, Lei Shi, Larry R. Masterson, Ritimukta Sarangi, Gianluigi Veglia, Burckhard Seelig

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a noncatalytic protein scaffold, we recently generated a new RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely new structure with substantially enhanced conformational dynamics, demonstrating that a primordial fold with suitable flexibility is sufficient to carry out enzymatic function.

Original languageEnglish (US)
Pages (from-to)81-83
Number of pages3
JournalNature Chemical Biology
Volume9
Issue number2
DOIs
StatePublished - Feb 1 2013

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