Structural polypeptides of the american (VR-2332) strain of porcine reproductive and respiratory syndrome virus

E. M. Bautista, J. J.M. Meulenberg, C. S. Choi, T. W. Molitor

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

The structural polypeptides of the isolate VR-2332 of porcine reproductive and respiratory syndrome virus were analyzed in sucrose gradient-purified virions. The virus had all average density of 1.15 g/cm3 and contained, by SDS-PAGE, three major polypeptides with apparent molecular weights of 15, 19 and 26-30 kDa, which were designated as nucleocapsid (N), matrix (M) and envelope (E), respectively. The predominant structural protein was N. N-glycosidase F digestion only affected E whereas O- glycosidase or endoglycosidase H digestion had no effect, suggesting that the viral glycoproteins contain only complex N-linked carbohydrates.

Original languageEnglish (US)
Pages (from-to)1357-1365
Number of pages9
JournalArchives of Virology
Volume141
Issue number7
DOIs
StatePublished - 1996

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