Structural features of the priming signal recognized by primase: Mutational analysis of the phage G4 origin of complementary DNA strand synthesis

Hiroshi Hiasa; Hiroshi Sakai; Tohru Komano; G. Nigel Godson

doi:10.1093/nar/18.16.4825

Structural features of the priming signal recognized by primase: Mutational analysis of the phage G4 origin of complementary DNA strand synthesis

Hiroshi Hiasa, Hiroshi Sakai, Tohru Komano, G. Nigel Godson

Pharmacology

Research output: Contribution to journal › Article › peer-review

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Abstract

45 mutations (insertion, deletion and base substitution) of the G4 Gori_c were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to synthesize pRNA in vitro using SSB and primase. The results demonstrate that the secondary structure and spacing of stem-loops I and III are essential for Gori_c activity and that the 5′-CTG-3′ sequence flanking stem-loop I is essential for initiation of pRNA synthesis.

Original language	English (US)
Pages (from-to)	4825-4831
Number of pages	7
Journal	Nucleic acids research
Volume	18
Issue number	16
DOIs	https://doi.org/10.1093/nar/18.16.4825
State	Published - Aug 25 1990

Bibliographical note

Funding Information:
We thank Dr. Nobuo Nomura for his communication and gifts of E. coli and phage strains. We also thank Drs. Hisao Masai, Kenneth Marians, Kinya Yoda and Tuneko Okazaki for their valuable discussions and communications prior to publication and Dr. Roger McMacken for providing us the purified primase.Ill This work was supported in part by Public Health Service Grant GM38292 to G.N.G.

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abstract = "45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to synthesize pRNA in vitro using SSB and primase. The results demonstrate that the secondary structure and spacing of stem-loops I and III are essential for Goric activity and that the 5′-CTG-3′ sequence flanking stem-loop I is essential for initiation of pRNA synthesis.",

author = "Hiroshi Hiasa and Hiroshi Sakai and Tohru Komano and Godson, {G. Nigel}",

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AU - Sakai, Hiroshi

AU - Komano, Tohru

AU - Godson, G. Nigel

N1 - Funding Information: We thank Dr. Nobuo Nomura for his communication and gifts of E. coli and phage strains. We also thank Drs. Hisao Masai, Kenneth Marians, Kinya Yoda and Tuneko Okazaki for their valuable discussions and communications prior to publication and Dr. Roger McMacken for providing us the purified primase.Ill This work was supported in part by Public Health Service Grant GM38292 to G.N.G.

PY - 1990/8/25

Y1 - 1990/8/25

N2 - 45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to synthesize pRNA in vitro using SSB and primase. The results demonstrate that the secondary structure and spacing of stem-loops I and III are essential for Goric activity and that the 5′-CTG-3′ sequence flanking stem-loop I is essential for initiation of pRNA synthesis.

AB - 45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to synthesize pRNA in vitro using SSB and primase. The results demonstrate that the secondary structure and spacing of stem-loops I and III are essential for Goric activity and that the 5′-CTG-3′ sequence flanking stem-loop I is essential for initiation of pRNA synthesis.

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Structural features of the priming signal recognized by primase: Mutational analysis of the phage G4 origin of complementary DNA strand synthesis

Abstract

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