Structural characterization of the poly(ethylene glycol) layer of sterically stabilized liposomes by means of FTIR spectroscopy

Z. Varga; J. Mihály; Sz Berényi; A. Bóta

doi:10.1016/j.eurpolymj.2013.02.025

Structural characterization of the poly(ethylene glycol) layer of sterically stabilized liposomes by means of FTIR spectroscopy

Z. Varga, J. Mihály, Sz Berényi, A. Bóta

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) combined with curve fitting procedures was used to characterize the poly(ethylene glycol) (PEG) layer of a pharmacologically relevant liposomal drug delivery system. The hydration of the polymer layer was described based on the ratio of the trans and gauche configurations of the C-O-C bond of the PEG molecules, which show a minimum at ∼6 mol% PEG-lipid molar ratio. The latter corresponds to highest bilayer stability, and coincides with the transition from the mushroom to the brush configuration of the PEG structure. This preliminary study showed that the ATR-FTIR technique is capable of qualitatively describing the hydration of the PEG-layer of stealth liposomes, which is in connection with the structure of the polymer on the liposomal surface.

Original language	English (US)
Pages (from-to)	2415-2421
Number of pages	7
Journal	European Polymer Journal
Volume	49
Issue number	9
DOIs	https://doi.org/10.1016/j.eurpolymj.2013.02.025
State	Published - Sep 2013
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Hungarian Scientific Research Fund (OTKA, Hungary) and the National Innovation Office (NIH, Hungary) under Grant agreement CNK-81056. The authors thank the suggestions of the anonymous referees, whose valuable comments helped to improve our manuscript.

Keywords

Fourier transform infrared spectroscopy
Hydration
Liposomes
Poly(ethylene glycol)
Steric stabilization

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10.1016/j.eurpolymj.2013.02.025

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title = "Structural characterization of the poly(ethylene glycol) layer of sterically stabilized liposomes by means of FTIR spectroscopy",

abstract = "Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) combined with curve fitting procedures was used to characterize the poly(ethylene glycol) (PEG) layer of a pharmacologically relevant liposomal drug delivery system. The hydration of the polymer layer was described based on the ratio of the trans and gauche configurations of the C-O-C bond of the PEG molecules, which show a minimum at ∼6 mol% PEG-lipid molar ratio. The latter corresponds to highest bilayer stability, and coincides with the transition from the mushroom to the brush configuration of the PEG structure. This preliminary study showed that the ATR-FTIR technique is capable of qualitatively describing the hydration of the PEG-layer of stealth liposomes, which is in connection with the structure of the polymer on the liposomal surface.",

keywords = "Fourier transform infrared spectroscopy, Hydration, Liposomes, Poly(ethylene glycol), Steric stabilization",

author = "Z. Varga and J. Mih{\'a}ly and Sz Ber{\'e}nyi and A. B{\'o}ta",

note = "Funding Information: This work was supported by the Hungarian Scientific Research Fund (OTKA, Hungary) and the National Innovation Office (NIH, Hungary) under Grant agreement CNK-81056. The authors thank the suggestions of the anonymous referees, whose valuable comments helped to improve our manuscript.",

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AU - Varga, Z.

AU - Mihály, J.

AU - Berényi, Sz

AU - Bóta, A.

N1 - Funding Information: This work was supported by the Hungarian Scientific Research Fund (OTKA, Hungary) and the National Innovation Office (NIH, Hungary) under Grant agreement CNK-81056. The authors thank the suggestions of the anonymous referees, whose valuable comments helped to improve our manuscript.

PY - 2013/9

Y1 - 2013/9

N2 - Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) combined with curve fitting procedures was used to characterize the poly(ethylene glycol) (PEG) layer of a pharmacologically relevant liposomal drug delivery system. The hydration of the polymer layer was described based on the ratio of the trans and gauche configurations of the C-O-C bond of the PEG molecules, which show a minimum at ∼6 mol% PEG-lipid molar ratio. The latter corresponds to highest bilayer stability, and coincides with the transition from the mushroom to the brush configuration of the PEG structure. This preliminary study showed that the ATR-FTIR technique is capable of qualitatively describing the hydration of the PEG-layer of stealth liposomes, which is in connection with the structure of the polymer on the liposomal surface.

AB - Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) combined with curve fitting procedures was used to characterize the poly(ethylene glycol) (PEG) layer of a pharmacologically relevant liposomal drug delivery system. The hydration of the polymer layer was described based on the ratio of the trans and gauche configurations of the C-O-C bond of the PEG molecules, which show a minimum at ∼6 mol% PEG-lipid molar ratio. The latter corresponds to highest bilayer stability, and coincides with the transition from the mushroom to the brush configuration of the PEG structure. This preliminary study showed that the ATR-FTIR technique is capable of qualitatively describing the hydration of the PEG-layer of stealth liposomes, which is in connection with the structure of the polymer on the liposomal surface.

KW - Fourier transform infrared spectroscopy

KW - Hydration

KW - Liposomes

KW - Poly(ethylene glycol)

KW - Steric stabilization

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JF - European Polymer Journal

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Structural characterization of the poly(ethylene glycol) layer of sterically stabilized liposomes by means of FTIR spectroscopy

Abstract

Bibliographical note

Keywords

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