Structural characterization of hemicellulosic polysaccharides isolated from bamboo (Phyllostachys pubescens Mazel)

Hong Peng, Jinsheng Zhang, Yuhuan Liu, Detao Liu, Ziping Yu, Yiqin Wan, Roger Ruan

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In the present work water soluble hemicellulosic polysaccharides HPW, 2% NaOH soluble hemicelluloses HPI and HPIII, and 5% KOH soluble fractions HPII and HPIV, were classically separated from bamboo (Phyllostachys pubescens Mazel) aged one year. In addition to sugar composition analysis, the hemicelluloses were also elucidated by GPC, FTIR, NMR and AFM techniques. Xylose (33.70-74.09%) and arabinose (25.03-59.04%) were the main neutral sugar, with ratios of arabinose to xylose being between 0.34 and 1.70. GPC measurement showed that the fractions HPW, HPII and HPIII precipitated in ethanol presented much broader molecular weight distribution. FTIR and NMR analysis suggested that the bamboo hemicelluloses could be mainly defined as arabinoxylans substituted by (1→2)-linked 4-O-methyl-α-D-glucuronic aid and acetyl group as side chains. AFM images of HPIII revealed a distribution of both island and rod aggregations when 0.01 mg/mL sample in 0.1 M NH 4Ac solution was deposited onto freshly cleared mica and air dried.

Original languageEnglish (US)
Pages (from-to)1855-1862
Number of pages8
JournalCurrent Organic Chemistry
Volume16
Issue number16
DOIs
StatePublished - Aug 9 2012

Keywords

  • AFM analysis
  • Bamboo
  • Hemicellulosic polysaccharides
  • Molecular weight distribution
  • NMR analysis
  • Structure
  • Sugar composition

Fingerprint

Dive into the research topics of 'Structural characterization of hemicellulosic polysaccharides isolated from bamboo (Phyllostachys pubescens Mazel)'. Together they form a unique fingerprint.

Cite this