A novel heteropolysaccharide from Grifola frondosa mycelia was extracted and purified using DEAE Sephadex A-50 and Sephadex G-200 chromatography. Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance (1H NMR and 13C NMR) spectroscopy were used to decipher the structure of the purified G. frondosa polysaccharide (GFP1). Chemical and spectral analysis revealed that GFP1, with an average molecular weight of 40.5 kDa, possessed a 1,6-β-d-glucan backbone with a single 1,3-α-d-fucopyranosyl side-branching unit. Enterovirus 71 (EV71) is the causative pathogen of hand-foot-and-mouth disease. GFP1 was tested for its anti-EV71 activity in cultured cells, which showed that EV71 viral replication was blocked and viral VP1 protein expression and genomic RNA synthesis were suppressed. Moreover, GFP1 exhibited apoptotic and other activities by suppressing the EV71-induced caspase-3 cleavage and IκBα down regulation. Our results demonstrate that the novel G. frondosa polysaccharide has antiviral activity, which could be valuable as a potentially new anti-EV71 therapeutic compound.
Bibliographical noteFunding Information:
This study was financially supported by the School-Enterprise Cooperation Major Projects of Fujian, China ( 2014N5007 ) to CZ and BL, FAFU grants ( XJQ201417 & 612014042 ) to CZ, and the Natural Science Foundation of Jiangsu Province, China ( BK20141078 ) to YJ. The project was also supported by the Mega Infectious Diseases Program from the Ministry of Science of Technology of China ( 2014ZX10004001-002 ) and a grant from the State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University ( KFGW-200902 ) to ZX.
© 2016 Published by Elsevier Ltd.
- Chemical properties
- Enterovirus 71
- Grifola frondosa polysaccharide
- Viral replication