Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7

Yajun Jiang, Chen Jie Zhou, Zi Ren Zhou, Meng Wu, Hong Yu Hu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ataxin-7 (Atx7) is a component of the nuclear transcription co-activator complex; its polyglutamine (polyQ) expansion may cause nuclear accumulation and recruit numerous proteins to the intranuclear inclusion bodies. Full-length R85 (R85FL) is such a protein sequestered by polyQ-expanded Atx7. Here, we report that Atx7 specifically interacts with the third SH3 domain (SH3C) of R85FL through its second portion of proline-rich region (PRR). NMR structural analysis of the SH3C domain and its complex with PRR revealed that SH3C contains a large negatively charged surface for binding with the RRTR motif of Atx7. Microscopy imaging demonstrated that sequestration of R85FL by the polyQ-expanded Atx7 in cell is mediated by this specific SH3C-PRR interaction, which is implicated in the pathogenesis of spinocerebellar ataxia 7.

Original languageEnglish (US)
Pages (from-to)2905-2911
Number of pages7
JournalFEBS Letters
Volume587
Issue number18
DOIs
StatePublished - Sep 17 2013

Fingerprint

src Homology Domains
Proline
Spinocerebellar Ataxias
Intranuclear Inclusion Bodies
Transcription
Structural analysis
Microscopy
Microscopic examination
Proteins
Nuclear magnetic resonance
Ataxin-7
ponsin
Imaging techniques
polyglutamine

Keywords

  • Ataxin-7
  • Inclusion body
  • Polyglutamine
  • Proline-rich region
  • R85FL/ponsin
  • SH3 domain

Cite this

Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. / Jiang, Yajun; Zhou, Chen Jie; Zhou, Zi Ren; Wu, Meng; Hu, Hong Yu.

In: FEBS Letters, Vol. 587, No. 18, 17.09.2013, p. 2905-2911.

Research output: Contribution to journalArticle

Jiang, Yajun ; Zhou, Chen Jie ; Zhou, Zi Ren ; Wu, Meng ; Hu, Hong Yu. / Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. In: FEBS Letters. 2013 ; Vol. 587, No. 18. pp. 2905-2911.
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AU - Hu, Hong Yu

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