Strontium- and peptide-modified silicate nanostructures for dual osteogenic and antimicrobial activity

Isha Mutreja, Dhiraj Kumar, Kami Hogan, Emily A Campbell, Kim Mansky, Conrado Aparicio

Research output: Contribution to journalArticlepeer-review

Abstract

Developing multifunctional nanostructures that promote bone repair while fighting infection is highly desirable in bone regenerative therapies. Previous efforts have focused on achieving one property or another by altering the chemical makeup of nanostructures or using growth factors or antibiotics. We present nanostructures with several simultaneous functional attributes including positive effects of strontium on bone formation and prevention of osteoclast differentiation along with incorporation of antimicrobial peptides (AMP) to prevent infection. To form these multifunctional nanostructures, mesoporous calcium silicate (CaMSN) was modified with high levels of strontium. For this, CaMSNs were either partially substituted (20 wt% Ca) or completely replaced with strontium (Sr) to form Sr-CaMSN or SrMSN. The mesoporous nature of these bioactive silicate nanostructures rendered a configuration for substantial AMP loading as well as their effective delivery. The physico-chemical and structural characterization of synthesized MSNs confirmed the mesoporous nature of the synthesized MSNs and their total surface area, pore size, pore volume and SBF-mediated bioactivity remained unaltered with the incorporation of Sr. However, biological evaluation confirmed that synthesized SrMSN upregulated osteogenic differentiation of mesenchymal stromal cells and significantly downregulated osteoclast differentiation. Also, the AMP-loaded MSNs prevented formation and growth of methicillin resistant Staphylococcus aureus (MRSA) biofilms. Thus, high Sr-containing AMP-loaded SrMSNs may combat MRSA-associated infection while promoting bone regeneration. The controlled availability of therapeutic Sr and AMP release as SrMSN degrade enables its potential application in bone tissue regeneration.

Original languageEnglish (US)
Article number212735
JournalBiomaterials Advances
Volume135
DOIs
StatePublished - Apr 22 2022

Bibliographical note

Funding Information:
The authors acknowledge Professor Sven Gorr from University of Minnesota for scientific discussions related to design of antibacterial studies and Prof Andrew Steins for providing access to the Quantachrome Autosorb-1 instrument. This work was supported by the Assistant Secretary of Defense for Health Affairs through the Peer Reviewed Orthopaedic Research Program (PRORP) under Applied Research Award No W81XWH-20-1-0563 (CA and IM). Parts of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF - Funded Materials Research Facilities Network (www.mrfn.org) via the MRSEC program. The Scheme was partially created with BioRender.

Funding Information:
The authors acknowledge Professor Sven Gorr from University of Minnesota for scientific discussions related to design of antibacterial studies and Prof Andrew Steins for providing access to the Quantachrome Autosorb-1 instrument. This work was supported by the Assistant Secretary of Defense for Health Affairs through the Peer Reviewed Orthopaedic Research Program (PRORP) under Applied Research Award No W81XWH-20-1-0563 (CA and IM). Parts of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF - Funded Materials Research Facilities Network ( www.mrfn.org ) via the MRSEC program. The Scheme was partially created with BioRender.

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

  • Anti-osteoclastogenic activity
  • Antimicrobial peptide
  • Bioactive strontium
  • Bone regeneration
  • Mesoporous nanostructures
  • Multifunctional system

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