Stringent control of FLP recombinase in Escherichia coli

Steven Bowden, Nagendra Prasad Palani, Igor G.L. Libourel

Research output: Contribution to journalArticle

Abstract

Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

Original languageEnglish (US)
Pages (from-to)52-54
Number of pages3
JournalJournal of Microbiological Methods
Volume133
DOIs
StatePublished - Feb 1 2017

Fingerprint

Synthetic Biology
Arabinose
Ribosomes
Molecular Biology
Binding Sites
Escherichia coli
Site-specific recombinase
FLP recombinase

Keywords

  • Escherichia coli, arabinose
  • Flp recombinase
  • Gene expression
  • Repression

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

Cite this

Stringent control of FLP recombinase in Escherichia coli. / Bowden, Steven; Palani, Nagendra Prasad; Libourel, Igor G.L.

In: Journal of Microbiological Methods, Vol. 133, 01.02.2017, p. 52-54.

Research output: Contribution to journalArticle

Bowden, Steven ; Palani, Nagendra Prasad ; Libourel, Igor G.L. / Stringent control of FLP recombinase in Escherichia coli. In: Journal of Microbiological Methods. 2017 ; Vol. 133. pp. 52-54.
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