Stringent control of FLP recombinase in Escherichia coli

Steven D. Bowden, Nagendra P. Palani, Igor G.L. Libourel

Research output: Contribution to journalArticle

Abstract

Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

Original languageEnglish (US)
Pages (from-to)52-54
Number of pages3
JournalJournal of Microbiological Methods
Volume133
DOIs
StatePublished - Feb 1 2017

Keywords

  • Escherichia coli, arabinose
  • Flp recombinase
  • Gene expression
  • Repression

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