Stringent control of FLP recombinase in Escherichia coli

Steven D. Bowden; Nagendra P. Palani; Igor G.L. Libourel

doi:10.1016/j.mimet.2016.12.018

Stringent control of FLP recombinase in Escherichia coli

Steven D. Bowden, Nagendra P. Palani, Igor G.L. Libourel

Research output: Contribution to journal › Article › peer-review

Abstract

Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

Original language	English (US)
Pages (from-to)	52-54
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	133
DOIs	https://doi.org/10.1016/j.mimet.2016.12.018
State	Published - Feb 1 2017

Keywords

Escherichia coli, arabinose
Flp recombinase
Gene expression
Repression

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10.1016/j.mimet.2016.12.018

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title = "Stringent control of FLP recombinase in Escherichia coli",

abstract = "Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.",

keywords = "Escherichia coli, arabinose, Flp recombinase, Gene expression, Repression",

author = "Bowden, {Steven D.} and Palani, {Nagendra P.} and Libourel, {Igor G.L.}",

year = "2017",

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doi = "10.1016/j.mimet.2016.12.018",

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T1 - Stringent control of FLP recombinase in Escherichia coli

AU - Bowden, Steven D.

AU - Palani, Nagendra P.

AU - Libourel, Igor G.L.

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N2 - Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

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KW - Escherichia coli, arabinose

KW - Flp recombinase

KW - Gene expression

KW - Repression

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ER -

Stringent control of FLP recombinase in Escherichia coli

Abstract

Keywords

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