Stretch-activated calcium signal propagation following mechanical stimulation of focal adhesions

Warren C. Ruder, Erica D. Pratt, Nailah Z. Brandy, David A. LaVan, Philip R. LeDuc, James F. Antaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cells translate environmental mechanical stimuli into biochemical reactions that govern a range of cellular processes such as proliferation, death and tissue matrix remodeling. Mechanical activation of individual focal adhesions formed between the cell and its environment directly correspond to several internal responses. Intracellular calcium concentration, [Ca2+] in, has been shown to profoundly change during force sensing. In order to understand this dynamic in cells, we compared calcium mobilization resulting from chemical stimulation and that resulting from mechanical stimulation. We have analyzed the response of fibroblasts to membrane displacements of over 5 μm resulting in eventual spikes in [Ca 2+]in. Our data initially indicates that fibroblasts may process mechanical calcium events in unique manner in comparison to other cell types. This finding has implications in a range of fields including mechanobiology and magnetics based activation.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages271-272
Number of pages2
ISBN (Print)0791847985, 9780791847985
DOIs
StatePublished - Jan 1 2007
Event2007 ASME Summer Bioengineering Conference, SBC 2007 - Keystone, CO, United States
Duration: Jun 20 2007Jun 24 2007

Publication series

NameProceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007

Other

Other2007 ASME Summer Bioengineering Conference, SBC 2007
CountryUnited States
CityKeystone, CO
Period6/20/076/24/07

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