TY - JOUR
T1 - Stress-dependent effects of platelet-derived growth factor-BB on fibroblast migration and traction differ in collagen and fibrin
AU - Shreiber, David I.
AU - Enever, Paul A J
AU - Tranquillo, Robert T
PY - 2000
Y1 - 2000
N2 - We used our novel assays of cell behavior in tissue equivalents to study the dose-response effects of PDGF-BB on RDF migration and traction in mechanically stressed and stress-free type I collagen and fibrin gels. PDGF-BB increased fibroblast migration significantly in all assays, but the effects on traction depended on the presence of stress and the nature of the ECM. PDGF-BB decreased fibroblast traction in stressed collagen gels, but increased traction in stress-free gels. No statistical conclusion could be inferred for stressed fibrin gels, and increasing PDGF-BB decreased traction in stress-free fibrin gels. These results demonstrate the complex response of fibroblasts to environmental cues, and point to opportunities to orchestrate cell behavior to affect the outcome of wound healing.
AB - We used our novel assays of cell behavior in tissue equivalents to study the dose-response effects of PDGF-BB on RDF migration and traction in mechanically stressed and stress-free type I collagen and fibrin gels. PDGF-BB increased fibroblast migration significantly in all assays, but the effects on traction depended on the presence of stress and the nature of the ECM. PDGF-BB decreased fibroblast traction in stressed collagen gels, but increased traction in stress-free gels. No statistical conclusion could be inferred for stressed fibrin gels, and increasing PDGF-BB decreased traction in stress-free fibrin gels. These results demonstrate the complex response of fibroblasts to environmental cues, and point to opportunities to orchestrate cell behavior to affect the outcome of wound healing.
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M3 - Article
AN - SCOPUS:14044266981
SN - 1071-6947
VL - 48
SP - 183
EP - 184
JO - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
JF - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
ER -