Stress-dependent effects of platelet-derived growth factor-BB on fibroblast migration and traction differ in collagen and fibrin

David I. Shreiber, Paul A J Enever, Robert T Tranquillo

Research output: Contribution to journalArticlepeer-review

Abstract

We used our novel assays of cell behavior in tissue equivalents to study the dose-response effects of PDGF-BB on RDF migration and traction in mechanically stressed and stress-free type I collagen and fibrin gels. PDGF-BB increased fibroblast migration significantly in all assays, but the effects on traction depended on the presence of stress and the nature of the ECM. PDGF-BB decreased fibroblast traction in stressed collagen gels, but increased traction in stress-free gels. No statistical conclusion could be inferred for stressed fibrin gels, and increasing PDGF-BB decreased traction in stress-free fibrin gels. These results demonstrate the complex response of fibroblasts to environmental cues, and point to opportunities to orchestrate cell behavior to affect the outcome of wound healing.

Original languageEnglish (US)
Pages (from-to)183-184
Number of pages2
JournalAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume48
StatePublished - Dec 1 2000

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