Streptococcus gordonii utilizes several distinct gene functions to recruit Porphyromonas gingivalis into a mixed community

Masae Kuboniwa, Gena D. Tribble, Chloe E. James, Ali O. Kilic, Lin Tao, Mark C Herzberg, Satoshi Shizukuishi, Richard J. Lamont

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Dental plaque biofilm formation proceeds through a developmental pathway initiated by the attachment of pioneer organisms, such as Streptococcus gordonii, to tooth surfaces. Through a variety of synergistic interactions, pioneer organisms facilitate the colonization of later arrivals including Porphyromonas gingivalis, a potential periodontal pathogen. We have investigated genes of S. gordonii required to support a heterotypic biofilm community with P. gingivalis. By screening a plasmid integration library of S. gordonii, genes were identified that are crucial for the accumulation of planktonic P. gingivalis cells into a multispecies biofilm. These genes were further investigated by specific mutation and complementation analyses. The biofilm-associated genes can be grouped into broad categories based on putative function as follows: (i) intercellular or intracellular signalling (cbe and spxB), (ii) cell wall integrity and maintenance of adhesive proteins (murE, msrA and atf), (iii) extracellular capsule biosynthesis (pgsA and atf), and (iv) physiology (gdhA, ccmA and ntpB). In addition, a gene for a hypothetical protein was identified. Biofilm visualization and quantification by confocal microscopy confirmed the role of these genes in the maturation of the multispecies community, including biofilm architectural development. The results suggest that S. gordonii governs the development of heterotypic oral biofilms through multiple genetic pathways.

Original languageEnglish (US)
Pages (from-to)121-139
Number of pages19
JournalMolecular Microbiology
Volume60
Issue number1
DOIs
StatePublished - Apr 1 2006

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Streptococcus gordonii
Porphyromonas gingivalis
Biofilms
Genes
Dental Plaque
Confocal Microscopy
Adhesives
Cell Wall
Libraries
Capsules
Tooth
Proteins
Plasmids
Maintenance
Mutation

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Streptococcus gordonii utilizes several distinct gene functions to recruit Porphyromonas gingivalis into a mixed community. / Kuboniwa, Masae; Tribble, Gena D.; James, Chloe E.; Kilic, Ali O.; Tao, Lin; Herzberg, Mark C; Shizukuishi, Satoshi; Lamont, Richard J.

In: Molecular Microbiology, Vol. 60, No. 1, 01.04.2006, p. 121-139.

Research output: Contribution to journalArticle

Kuboniwa, Masae ; Tribble, Gena D. ; James, Chloe E. ; Kilic, Ali O. ; Tao, Lin ; Herzberg, Mark C ; Shizukuishi, Satoshi ; Lamont, Richard J. / Streptococcus gordonii utilizes several distinct gene functions to recruit Porphyromonas gingivalis into a mixed community. In: Molecular Microbiology. 2006 ; Vol. 60, No. 1. pp. 121-139.
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