Streptococci dominate the diverse flora within buccal cells

Joel D Rudney, R. Chen, G. Zhang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Previously, we reported that intracellular Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. In this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify Fusobacterium nucleatum, Prevotella intermedia, oral Campylobacter species, Eikenella corrodens, Treponema denticola, Gemella haemolysans, Granulicatella adiacens, and total streptococci within buccal epithelial cells. All those species were found within buccal cells. In every case, species recognized by green-labeled species-specific probes were accompanied by other bacteria recognized only by a red-labeled universal probe. Streptococci appeared to be a major component of the polymicrobial intracellular flora, being present at a level from one to two logs greater than the next most common species (G. adiacens). This is similar to what is observed in oral biofilms, where diverse species interact in complex communities that often are dominated by streptococci.

Original languageEnglish (US)
Pages (from-to)1165-1171
Number of pages7
JournalJournal of Dental Research
Volume84
Issue number12
DOIs
StatePublished - Dec 1 2005

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Cheek
Streptococcus
Gemella
Eikenella corrodens
Epithelial Cells
Treponema denticola
Prevotella intermedia
Fusobacterium nucleatum
Aggregatibacter actinomycetemcomitans
Porphyromonas gingivalis
Campylobacter
Biofilms
Bacteria

Keywords

  • Bacterial invasion
  • Buccal cells
  • Campylobacter
  • Eikenella corrodens
  • Fusobacterium nucleatum
  • Gemella haemolysans
  • Granulicatella adiacens
  • Prevotella intermedia
  • Streptococcus
  • Treponema denticola

Cite this

Streptococci dominate the diverse flora within buccal cells. / Rudney, Joel D; Chen, R.; Zhang, G.

In: Journal of Dental Research, Vol. 84, No. 12, 01.12.2005, p. 1165-1171.

Research output: Contribution to journalArticle

Rudney, Joel D ; Chen, R. ; Zhang, G. / Streptococci dominate the diverse flora within buccal cells. In: Journal of Dental Research. 2005 ; Vol. 84, No. 12. pp. 1165-1171.
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