Streptococcal Cell Wall-Induced Arthritis: Requirements for Neutrophils, P-Selectin, Intercellular Adhesion Molecule-1, and Macrophage-lnflammatory Protein-2

Ralph C. Schimmer, Denis J. Schrier, Craig M. Flory, James Dykens, David K.L. Tung, Peer B. Jacobson, Hans P. Friedl, Mary Carol Conroy, Beatrice Beck Schimmer, Peter A. Ward

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42 Scopus citations

Abstract

Immune arthritis in rat ankle joints was induced by intra-articular injection of streptococcal cell wall extract (SCW), followed 21 days later by i.v. injection of SCW. This results in a monoarticular arthritis characterized by an influx of neutrophils and mononuclear cells, a 35-fold increase in urinary excretion of 8-hydroxy-deoxyguanosine (8-OH-dGUA; an index of free radical production), ankle edema, and joint damage/destruction. Neutrophil depletion substantially reduced the intensity of ankle edema. Ab-induced blockade of P-selectin or ICAM-1 also reduced the intensity of ankle edema and the influx of neutrophils. Blockade of TNF-α or IL-1 resulted in nearly complete and persistent reduction in ankle edema and profound reductions in the accumulation of neutrophils and mononuclear cells in affected joints. Finally, blocking of macrophage-inflammatory protein-2 reduced ankle edema and neutrophil accumulation during the first 2 days after i.v. challenge with SCW. These data indicate that SCW-induced arthritis is neutrophil dependent and that the recruitment of neutrophils and subsequent joint edema requires ICAM-1, P-selectin, and macrophage-inflammatory protein-2, as well as TNF-α and IL-1.

Original languageEnglish (US)
Pages (from-to)4103-4108
Number of pages6
JournalJournal of Immunology
Volume159
Issue number8
StatePublished - Oct 15 1997
Externally publishedYes

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