Streptococcal cell wall-induced arthritis: Requirements for IL-4, IL- 10, IFN-γ, and monocyte chemoattractant protein-1

Ralph C. Schimmer, Denis J. Schrier, Craig M. Flory, Keith D. Laemont, David Tung, Alan L. Metz, Hans P. Friedl, Mary Carol Conroy, Jeffrey S. Warren, Beatrice Beck, Peter A. Ward

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Intra-articular injection of streptococcal cell wall Ag followed by i.v. challenge ('reactivation') results in a destructive lymphocyte-dependent monoarticular arthritis. To further define the role of immune mechanisms in the model, Abs to Th1 and Th2-related cytokines were evaluated. Treatment of rats with antibodies to IL-4 reduced swelling, while treatment with anti-IL- 10 or anti-IFN-γ either had no effect or slightly enhanced the inflammatory response. These results suggest that Th-2 immune mechanisms may be, at least in part, operative in the model. To more precisely define the role of IL-4, the effects of anti-IL-4 on monocyte chemoattractant protein-1 (MCP-1) expression were evaluated. Initial studies demonstrated that mRNA (as determined by in situ hybridization) and protein (as determined by immunofluorescence) for MCP-1 were detectable in inflamed synovial tissue in a time-dependent manner. Anti-IL-4 treatment significantly reduced the expression of mRNA for MCP-1 24 and 72 h after reactivation. In addition, anti-MCP-1 inhibited swelling and reduced influx of 111In-labeled T cells. These data suggest that the reactivation model of streptococcal cell wall Ag- induced arthritis is Th-2 dependent, and that an inter-relationship exists between IL-4 and the expression of MCP-1.

Original languageEnglish (US)
Pages (from-to)1466-1471
Number of pages6
JournalJournal of Immunology
Volume160
Issue number3
StatePublished - Feb 1 1998
Externally publishedYes

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