Streak Fluorescence Lifetime Imaging Microscopy. A Novel Technology for Quantitative FRET Imaging

V. Krishnan Ramanujan, Jian Hua Zhang, Victoria E. Centonze, Brian Herman

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

This chapter provides a comprehensive description of this streak camera-based fluorescence lifetime imaging microscopy (FLIM) imaging (StreakFLIM) system and contrasts its performance with that of the other currently available time-domain FLIM systems. The FLIM methods are relatively insensitive to artifacts that can affect spectroscopic characteristics of fluorophores and can provide distinct lifetimes characteristic of the individual fluorophores attached to each protein (for example, donor and acceptor). The main component of the StreakFLIM system is the streak camera, also known as streakscope, a device that can measure ultrafast light phenomena with a temporal resolution as low as 2 picoseconds (ps). In addition to intensity and time information, the streakscope can be used to obtain either spatial or wavelength information. The streak camera has good radiant sensitivity over a wide bandwidth of wavelengths, making it suitable for both single-photon and multiphoton imaging applications without compromising detection sensitivity. While in the conventional time-domain FLIM, multigate detection with a gated microchannel plate image intensifier and a charge-coupled device (CCD) imaging camera is used.

Original languageEnglish (US)
Title of host publicationMolecular Imaging
PublisherElsevier Inc.
Pages227-238
Number of pages12
ISBN (Print)9780195177206
DOIs
StatePublished - Dec 1 2005

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Optical Imaging
Microscopy
Microscopic examination
Fluorescence
Technology
Streak cameras
Imaging techniques
Fluorophores
Equipment and Supplies
Photons
Artifacts
Wavelength
Microchannels
Charge coupled devices
Light
Imaging systems
Cameras
Bandwidth
Proteins

Cite this

Ramanujan, V. K., Zhang, J. H., Centonze, V. E., & Herman, B. (2005). Streak Fluorescence Lifetime Imaging Microscopy. A Novel Technology for Quantitative FRET Imaging. In Molecular Imaging (pp. 227-238). Elsevier Inc.. https://doi.org/10.1016/B978-019517720-6.50021-3

Streak Fluorescence Lifetime Imaging Microscopy. A Novel Technology for Quantitative FRET Imaging. / Ramanujan, V. Krishnan; Zhang, Jian Hua; Centonze, Victoria E.; Herman, Brian.

Molecular Imaging. Elsevier Inc., 2005. p. 227-238.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ramanujan, V. Krishnan ; Zhang, Jian Hua ; Centonze, Victoria E. ; Herman, Brian. / Streak Fluorescence Lifetime Imaging Microscopy. A Novel Technology for Quantitative FRET Imaging. Molecular Imaging. Elsevier Inc., 2005. pp. 227-238
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