Strategies and technical challenges in allele level Class II typing in 2578 bone marrow transplantation donor-recipient pairs

T. M. Williams, T. Winden, M. Setterholm, C. A. Vierra-Green, S. Spellman, S. Flesch, Z. Awdeh, L. A. Baxter-Lowe, A. B. Begovich, M. Fernandez-Vina, J. Hegland, C. K. Hurley, D. Johnson, H. Noreen, M. Salazar, B. Schmeckpeper, E. J. Yunis

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Human leukocyte antigen typing of 2578 donor-recipient pairs whose transplantation was facilitated by the National Marrow Donor Program allowed for an in-depth analysis of the accuracy of high-volume allele level testing data. The methods employed provided allele level typing at DRB1/3/5, DQA1, DQB1, DPA1, and DPB1 using sequence-specific oligonucleotide probe hybridization (SSOPH), polymerase chain reaction (PCR) restriction fragment length polymorphism analysis, sequence specific PCR, and direct sequence-based typing (SBT). Each typing was independently tested by two laboratories in Phase 1, and in subsequent phases targeted samples were typed in duplicate by SBT to monitor typing quality. Comparison with prior transplant center typing was also evaluated. SSOPH detected discrepancies ranged from 0.6% at DPB1 to 5.1% at DQB1 in Phase 1. The majority of discrepancies, 62%, resulted from human error such as sample handling, result interpretation, or clerical errors. Alleles that are frequently discrepant have been identified in this predominantly white population.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalHuman Immunology
Issue number4-5
StatePublished - Apr 2008

Bibliographical note

Funding Information:
This work was facilitated by the staff of each of the participating laboratories, the NMDP, and the Blood Systems Research Institute. This research was supported by funding from the NMDP and the Office of Naval Research Grants N00014-93-1-0658 and N00014-95-1-0055 to the National Marrow Donor Program. The views expressed in this article do not reflect the official policy or position of the Department of the Navy, the Department of Defense, or the U.S. government.


  • Bone marrow transplantation
  • HLA antigens
  • Histocompatibility antigens class II


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