We tested the ability of seven MCF-7 strains to undergo DNA fragmentation, as measured by DNA laddering, following doxorubicin-induced apoptosis. Four strains were found to undergo DNA laddering while three were not. All strains were inhibited by doxorubicin, although sensitivity differed. Finally, we show by detection of sub-G1 DNA that doxorubicin induced the same fold increase in apoptosis in MCF-7-ATCC, which did not ladder, and MCF-7-MG, which did ladder. Therefore, detection of DNA ladders is not an accurate indicator of apoptosis in MCF-7 cells as fragmentation of DNA appears to vary between strains. Copyright (C) 1999 Elsevier Science Ireland Ltd.
Bibliographical noteFunding Information:
The authors wish to acknowledge Dr. Adrian Lee for critical review of this manuscript. This work was supported by Public Health Service (PHS) grant RO1CA74284 and PHS Cancer Center Support grant P30CA54174 (DY), and Department of Defense grant DAMD1798188339 (JLG).
Copyright 2007 Elsevier B.V., All rights reserved.
- Breast cancer
- DNA laddering