Stimulation with Concanavalin-A induces IL-17 production by Canine peripheral T cells

Michelle G Ritt, Beth A. Lindborg, Timothy D O'Brien, Joseph Bisignano, Jaime Modiano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The characteristics of canine IL-17-producing cells are incompletely understood. Expression of mRNA encoding orthologs of IL-17 and the IL-17 receptor has been documented in tissues from dogs with arthritis, inflammatory bowel disease, and lymphoma; however, no associations have been found between IL-17 gene expression and disease phenotype in these conditions. Robust assessment of the role of IL-17-producing cells in dogs will require measuring the frequency of these cells in health and disease in balance with other lymphocyte subsets. The aim of this study was to confirm that the T-cell IL-17 response in dogs is evolutionarily conserved. Canine peripheral blood mononuclear cells were stimulated with Concanavalin A with or without polarizing cytokines. We used a canine specific IL-17 ELISA and flow cytometry to identify IL-17-producing T cells. Accumulation of intracellular IL-17 was observed in stimulated CD4 and CD8 T cells. The addition of pro-inflammatory cytokines appeared to enhance polarization of canine CD4 T cells to the Th17 phenotype. Conversely, the addition of IL-2 in the presence of TGF-β resulted in expansion of Treg cells. We conclude that canine IL-17-producing cells behave similarly to those from humans and mice when stimulated with mitogens and polarized with pro-inflammatory or immune regulatory cytokines.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalVeterinary Sciences
Volume2
Issue number2
DOIs
StatePublished - Jun 1 2015

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Interleukin-17
concanavalin A
Concanavalin A
Canidae
T-lymphocytes
T-Lymphocytes
dogs
cytokines
Dogs
Cytokines
cells
Interleukin-17 Receptors
phenotype
Phenotype
inflammatory bowel disease
arthritis
mononuclear leukocytes
interleukin-2
lymphoma
Lymphocyte Subsets

Keywords

  • Canine
  • Cytokine
  • Flow cytometry
  • T Lymphocytes

Cite this

Stimulation with Concanavalin-A induces IL-17 production by Canine peripheral T cells. / Ritt, Michelle G; Lindborg, Beth A.; O'Brien, Timothy D; Bisignano, Joseph; Modiano, Jaime.

In: Veterinary Sciences, Vol. 2, No. 2, 01.06.2015, p. 43-51.

Research output: Contribution to journalArticle

Ritt, Michelle G ; Lindborg, Beth A. ; O'Brien, Timothy D ; Bisignano, Joseph ; Modiano, Jaime. / Stimulation with Concanavalin-A induces IL-17 production by Canine peripheral T cells. In: Veterinary Sciences. 2015 ; Vol. 2, No. 2. pp. 43-51.
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