Membranes and solubilized, reconstituted membranes from Moloney-infected tumors MBL-2 have been used to stimulate in vitro secondary cytolytic T lymphocytes (CTL) in C57BL/6 mice primed with Moloney murine sarcoma virus. Membranes are shown to stimulate the generation of Moloney-specific and H-2 restricted CTL. Stimulation with solubilized, reconstituted membranes required the presence of rat lymphocyte Con A supernatant (containing interleukin 2) during culture. Reconstituted membranes made in the presence of a detergent-insoluble fraction from the plasma membrane were able to stimulate a response in the absence of Con A supernatant.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Immunology|
|State||Published - 1983|