Stereochemistry- and concentration-dependent effects of phosphatidylserine enrichment on platelet function

Audrey F. Meyer, Sarah M. Gruba, Donghyuk Kim, Ben M. Meyer, Secil Koseoglu, Joseph J. Dalluge, Christy L. Haynes

Research output: Contribution to journalArticle

Abstract

Platelets are small (1–2 μm in diameter), circulating anuclear cell fragments with important roles in hemostasis and thrombosis that provide an excellent platform for studying the role of membrane components in cellular communication. Platelets use several forms of communication including exocytosis of three distinct granule populations, formation of bioactive lipid mediators, and shape change (allowing for adhesion). This work explores the role of stereochemistry and concentration of exogenous phosphatidylserine (PS) on platelet exocytosis and adhesion. PS, most commonly found in the phosphatidyl-L-serine (L-PS) form, is exposed on the outer leaflet of the cell membrane after the platelet is activated. Knowledge about the impact of exogenous phosphatidylserine on cell-to-cell communication is limited (particularly concentration and stereochemistry effects). This study found that platelets incubated in L-PS or phosphatidyl-D-serine (D-PS) are enriched to the same extent with their respective incubated PS. All levels of L-PS enrichment also showed an increase in platelet cholesterol, but only the 50 μM D-PS incubation showed an increase in cholesterol. The uptake of D-PS induced the secretion of granules and manufactured platelet activating factor (PAF) in otherwise unstimulated platelets. The uptake of L-PS had a greater impact on platelet stimulation by decreasing both the amount of δ-granule secretion and the amount of PAF that was manufactured.

Original languageEnglish (US)
Pages (from-to)1381-1387
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1859
Issue number8
DOIs
StatePublished - Aug 1 2017

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Stereochemistry
Phosphatidylserines
Platelets
Blood Platelets
Platelet Activating Factor
Exocytosis
Serine
Adhesion
Cholesterol
Cellular radio systems
Communication
L Forms
Cell membranes
Hemostasis
Cell Communication
Thrombosis

Keywords

  • Exocytosis
  • Phosphatidylserine
  • Platelet

Cite this

Stereochemistry- and concentration-dependent effects of phosphatidylserine enrichment on platelet function. / Meyer, Audrey F.; Gruba, Sarah M.; Kim, Donghyuk; Meyer, Ben M.; Koseoglu, Secil; Dalluge, Joseph J.; Haynes, Christy L.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1859, No. 8, 01.08.2017, p. 1381-1387.

Research output: Contribution to journalArticle

Meyer, Audrey F. ; Gruba, Sarah M. ; Kim, Donghyuk ; Meyer, Ben M. ; Koseoglu, Secil ; Dalluge, Joseph J. ; Haynes, Christy L. / Stereochemistry- and concentration-dependent effects of phosphatidylserine enrichment on platelet function. In: Biochimica et Biophysica Acta - Biomembranes. 2017 ; Vol. 1859, No. 8. pp. 1381-1387.
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