We demonstrate for the first time the applicability of multimodal nonlinear optical (NLO) microscopy to the interrogation of stented coronary arteries under different diet and stent deployment conditions. Bare metal stents and Taxus drug-eluting stents (DES) were placed in coronary arteries of Ossabaw pigs of control and atherogenic diet groups. Multimodal NLO imaging was performed to inspect changes in arterial structures and compositions after stenting. Sum frequency generation, one of the multimodalities, was used for the quantitative analysis of collagen content in the peristent and in-stent artery segments of both pig groups. Atherogenic diet increased lipid and collagen in peristent segments. In-stent segments showed decreased collagen expression in neointima compared to media. Deployment of DES in atheromatous arteries inhibited collagen expression in the arterial media.
Bibliographical noteFunding Information:
This work was supported by a NIH R01 Grant No. EB007243 to J. X. C., NIH Grant Nos. RR013223 and HL062552 to M.S., and the Comparative Medicine Program of Purdue and Indiana University School of Medicine and the Summer Research Program in Academic Medicine program of Indiana University School of Medicine. We thank Dr. Eric A. Mokelke of Boston Scientific, Inc., for the gift of Taxus stents.
- Ossabaw miniature swine
- coronary arteries
- drug eluting stents
- multimodal nonlinear optical microscopy