Statherin: A major boundary lubricant of human saliva

William H. Douglas; Ernest S. Reeh; Narayanan Ramasubbu; Periathamby A. Raj; Krishna K. Bhandary; Michael J. Levine

doi:10.1016/S0006-291X(05)81259-8

Statherin: A major boundary lubricant of human saliva

William H. Douglas, Ernest S. Reeh, Narayanan Ramasubbu, Periathamby A. Raj, Krishna K. Bhandary, Michael J. Levine

Biomaterials

Research output: Contribution to journal › Article › peer-review

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Abstract

The lubricating properties of human submandibular-sublingual salivary fractions were examined using a servohydraulic model of mandibular movement. Fractions containing statherin exhibited a strong tendency to boundary lubrication. The lubricity of purified statherin was confirmed and compared to the amphipathic molecules gramacidin S and sodium dodecyl sulfate. Contact angle measurements of statherin paralleled the other amphipathic molecules. The helical content of statherin increased in trifluoroethanol indicating the presence of amphipathic helical regions. CD studies and hydrophobic moment calculations indicated that statherin adopts an amphipathic helical conformation at the N-terminus. An energy-minimized model of the polar N-terminal residues 1-15 suggested that this domain could be positioned in space to interact with a hydroxyapatite substrate. These data imply that under appropriate conditions statherin may display an amphipathic nature which enables it to function as a boundary lubricant on enamel.

Original language	English (US)
Pages (from-to)	91-97
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	180
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(05)81259-8
State	Published - Oct 15 1991

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Funding Information:
This research was supported by the USPHS grant DE08240.

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title = "Statherin: A major boundary lubricant of human saliva",

abstract = "The lubricating properties of human submandibular-sublingual salivary fractions were examined using a servohydraulic model of mandibular movement. Fractions containing statherin exhibited a strong tendency to boundary lubrication. The lubricity of purified statherin was confirmed and compared to the amphipathic molecules gramacidin S and sodium dodecyl sulfate. Contact angle measurements of statherin paralleled the other amphipathic molecules. The helical content of statherin increased in trifluoroethanol indicating the presence of amphipathic helical regions. CD studies and hydrophobic moment calculations indicated that statherin adopts an amphipathic helical conformation at the N-terminus. An energy-minimized model of the polar N-terminal residues 1-15 suggested that this domain could be positioned in space to interact with a hydroxyapatite substrate. These data imply that under appropriate conditions statherin may display an amphipathic nature which enables it to function as a boundary lubricant on enamel.",

author = "Douglas, {William H.} and Reeh, {Ernest S.} and Narayanan Ramasubbu and Raj, {Periathamby A.} and Bhandary, {Krishna K.} and Levine, {Michael J.}",

note = "Funding Information: This research was supported by the USPHS grant DE08240.",

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AU - Douglas, William H.

AU - Reeh, Ernest S.

AU - Ramasubbu, Narayanan

AU - Raj, Periathamby A.

AU - Bhandary, Krishna K.

AU - Levine, Michael J.

N1 - Funding Information: This research was supported by the USPHS grant DE08240.

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Y1 - 1991/10/15

N2 - The lubricating properties of human submandibular-sublingual salivary fractions were examined using a servohydraulic model of mandibular movement. Fractions containing statherin exhibited a strong tendency to boundary lubrication. The lubricity of purified statherin was confirmed and compared to the amphipathic molecules gramacidin S and sodium dodecyl sulfate. Contact angle measurements of statherin paralleled the other amphipathic molecules. The helical content of statherin increased in trifluoroethanol indicating the presence of amphipathic helical regions. CD studies and hydrophobic moment calculations indicated that statherin adopts an amphipathic helical conformation at the N-terminus. An energy-minimized model of the polar N-terminal residues 1-15 suggested that this domain could be positioned in space to interact with a hydroxyapatite substrate. These data imply that under appropriate conditions statherin may display an amphipathic nature which enables it to function as a boundary lubricant on enamel.

AB - The lubricating properties of human submandibular-sublingual salivary fractions were examined using a servohydraulic model of mandibular movement. Fractions containing statherin exhibited a strong tendency to boundary lubrication. The lubricity of purified statherin was confirmed and compared to the amphipathic molecules gramacidin S and sodium dodecyl sulfate. Contact angle measurements of statherin paralleled the other amphipathic molecules. The helical content of statherin increased in trifluoroethanol indicating the presence of amphipathic helical regions. CD studies and hydrophobic moment calculations indicated that statherin adopts an amphipathic helical conformation at the N-terminus. An energy-minimized model of the polar N-terminal residues 1-15 suggested that this domain could be positioned in space to interact with a hydroxyapatite substrate. These data imply that under appropriate conditions statherin may display an amphipathic nature which enables it to function as a boundary lubricant on enamel.

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Statherin: A major boundary lubricant of human saliva

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