States and challenges for high-value biohythane production from waste biomass by dark fermentation technology

Zhidan Liu, Chong Zhang, Yuan Lu, Xiao Wu, Lang Wang, Linjun Wang, Bing Han, Xin Hui Xing

Research output: Contribution to journalArticlepeer-review

195 Scopus citations


Hythane (H2+CH4) has attracted growing attention due to its versatile advantages as, for instance vehicle fuel. Biohythane consisting of biohydrogen and biomethane via two-stage fermentation is a potential high-value solution for the valorization of waste biomass resources and probably an alternative to the fossil based hythane. However, the significance and application potential of biohythane have not yet been fully recognized. This review focuses on the progress of biohydrogen and subsequent biomethane fermentation in terms of substrate, microbial consortium, reactor configuration, as well as the H2/CH4 ratio from the perspective of the feasibility of biohythane production in the past ten years. The current paper also covers how controls of the microbial consortium and bioprocess, system integration influence the biohythane productivity. Challenges and perspectives on biohythane technology will finally be addressed. This review provides a state-of-the-art technological insight into biohythane production by two-stage dark fermentation from biomass.

Original languageEnglish (US)
Pages (from-to)292-303
Number of pages12
JournalBioresource Technology
StatePublished - May 2013

Bibliographical note

Funding Information:
This work was supported by the Project of the National Basic Research Program of China (973 Plan) ( 2011CB707404 ), NSFC-JST Cooperative Research Project ( 21161140328 ), NSFC project ( 21106080 ) and Chinese Universities Scientific Fund ( 2012RC030 ).


  • Biohydrogen
  • Biohythane
  • Dark fermentation
  • Two-stage production
  • Waste biomass


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