State of the art in preservation of fluid biospecimens

Allison Hubel, Alptekin Aksan, Amy P Skubitz, Christine H Wendt, Xiao Zhong

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Fluid biospecimens (blood, serum, urine, saliva, cerebrospinal fluid and bronchial lavage fluid) contain not only cells and subcellular components, but also proteins, enzymes, lipids, metabolites, and peptides, which are utilized as biomarkers. Availability of high-quality biospecimens is a requirement for biomarker discovery. The quality of the biospecimens depends upon preanalytical variables (ie, collection and processing techniques, freeze/thaw stability, and storage stability), which account for >60%-90% of the diagnostic errors. Currently, millions of fluid biospecimens are stored in hundreds of biorepositories across the nation, and tens of thousands of new biospecimens are added to the pool daily. Specimen stabilization is imperative, because fluid biospecimens degrade quickly when kept untreated at room temperature. Achieving a high-quality fluid biospecimen requires understanding the effects of storage processing parameters (eg, freezing and thawing as well as cryo-/lyoprotectant additives) and storage conditions on biomarkers contained within the biospecimens. In this article, we will discuss the main issues related to the stabilization of specific biofluids by reviewing (a) the current preservation and storage practices applied in biobanks/biorepositories and (b) the sensitivity of certain biomarkers to current storage techniques.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalBiopreservation and Biobanking
Volume9
Issue number3
DOIs
StatePublished - Sep 1 2011

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Biomarkers
Fluids
Stabilization
Bronchoalveolar Lavage Fluid
Cerebrospinal fluid
Cellular Structures
Diagnostic Errors
Saliva
Thawing
Freezing
Cerebrospinal Fluid
Metabolites
Processing
Urine
Lipids
Blood
Peptides
Temperature
Availability
Enzymes

Cite this

State of the art in preservation of fluid biospecimens. / Hubel, Allison; Aksan, Alptekin; Skubitz, Amy P; Wendt, Christine H; Zhong, Xiao.

In: Biopreservation and Biobanking, Vol. 9, No. 3, 01.09.2011, p. 237-244.

Research output: Contribution to journalArticle

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