Standard methods for American foulbrood research

Dirk C. De Graaf; Adriana M. Alippi; Karina Antúnez; Katherine A. Aronstein; Giles Budge; Dieter De Koker; Lina De Smet; Douglas W. Dingman; Jay D. Evans; Leonard J. Foster; Anne Fünfhaus; Eva Garcia-Gonzalez; Aleš Gregorc; Hannelie Human; K. Daniel Murray; Bach Kim Nguyen; Lena Poppinga; Marla Spivak; Dennis Van Engelsdorp; Selwyn Wilkins; Elke Genersch

doi:10.3896/IBRA.1.52.1.11

Standard methods for American foulbrood research

Dirk C. De Graaf, Adriana M. Alippi, Karina Antúnez, Katherine A. Aronstein, Giles Budge, Dieter De Koker, Lina De Smet, Douglas W. Dingman, Jay D. Evans, Leonard J. Foster, Anne Fünfhaus, Eva Garcia-Gonzalez, Aleš Gregorc, Hannelie Human, K. Daniel Murray, Bach Kim Nguyen, Lena Poppinga, Marla Spivak, Dennis Van Engelsdorp, Selwyn WilkinsElke Genersch

Entomology

Research output: Contribution to journal › Review article › peer-review

125 Scopus citations

Abstract

American foulbrood is one of the most devastating diseases of the honey bee. It is caused by the spore-forming, Gram-positive rod-shaped bacterium Paenibacillus larvae. The recent updated genome assembly and annotation for this pathogen now permits in-depth molecular studies. In this paper, selected techniques and protocols for American foulbrood research are provided, mostly in a recipe-like format that permits easy implementation in the laboratory. Topics covered include: working with Paenibacillus larvae, basic microbiological techniques, experimental infection, and "'omics" and other sophisticated techniques. Further, this chapter covers other technical information including biosafety measures to guarantee the safe handling of this pathogen.

Original language	English (US)
Journal	Journal of Apicultural Research
Volume	52
Issue number	1
DOIs	https://doi.org/10.3896/IBRA.1.52.1.11
State	Published - 2013

Keywords

American foulbrood
BEEBOOK
Brood
COLOSS
Disease
Honey bee
Paenibacillus larvae
Pathogen
Technique

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10.3896/IBRA.1.52.1.11

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De Graaf, D. C., Alippi, A. M., Antúnez, K., Aronstein, K. A., Budge, G., De Koker, D., De Smet, L., Dingman, D. W., Evans, J. D., Foster, L. J., Fünfhaus, A., Garcia-Gonzalez, E., Gregorc, A., Human, H., Murray, K. D., Nguyen, B. K., Poppinga, L., Spivak, M., Van Engelsdorp, D., ... Genersch, E. (2013). Standard methods for American foulbrood research. Journal of Apicultural Research, 52(1). https://doi.org/10.3896/IBRA.1.52.1.11

De Graaf, DC, Alippi, AM, Antúnez, K, Aronstein, KA, Budge, G, De Koker, D, De Smet, L, Dingman, DW, Evans, JD, Foster, LJ, Fünfhaus, A, Garcia-Gonzalez, E, Gregorc, A, Human, H, Murray, KD, Nguyen, BK, Poppinga, L, Spivak, M, Van Engelsdorp, D, Wilkins, S & Genersch, E 2013, 'Standard methods for American foulbrood research', Journal of Apicultural Research, vol. 52, no. 1. https://doi.org/10.3896/IBRA.1.52.1.11

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AU - De Koker, Dieter

AU - De Smet, Lina

AU - Dingman, Douglas W.

AU - Evans, Jay D.

AU - Foster, Leonard J.

AU - Fünfhaus, Anne

AU - Garcia-Gonzalez, Eva

AU - Gregorc, Aleš

AU - Human, Hannelie

AU - Murray, K. Daniel

AU - Nguyen, Bach Kim

AU - Poppinga, Lena

AU - Spivak, Marla

AU - Van Engelsdorp, Dennis

AU - Wilkins, Selwyn

AU - Genersch, Elke

PY - 2013

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KW - COLOSS

KW - Disease

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KW - Paenibacillus larvae

KW - Pathogen

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Standard methods for American foulbrood research

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