Standard methods for American foulbrood research

Dirk C. De Graaf, Adriana M. Alippi, Karina Antúnez, Katherine A. Aronstein, Giles Budge, Dieter De Koker, Lina De Smet, Douglas W. Dingman, Jay D. Evans, Leonard J. Foster, Anne Fünfhaus, Eva Garcia-Gonzalez, Aleš Gregorc, Hannelie Human, K. Daniel Murray, Bach Kim Nguyen, Lena Poppinga, Marla Spivak, Dennis Van Engelsdorp, Selwyn WilkinsElke Genersch

Research output: Contribution to journalReview article

65 Scopus citations

Abstract

American foulbrood is one of the most devastating diseases of the honey bee. It is caused by the spore-forming, Gram-positive rod-shaped bacterium Paenibacillus larvae. The recent updated genome assembly and annotation for this pathogen now permits in-depth molecular studies. In this paper, selected techniques and protocols for American foulbrood research are provided, mostly in a recipe-like format that permits easy implementation in the laboratory. Topics covered include: working with Paenibacillus larvae, basic microbiological techniques, experimental infection, and "'omics" and other sophisticated techniques. Further, this chapter covers other technical information including biosafety measures to guarantee the safe handling of this pathogen.

Original languageEnglish (US)
JournalJournal of Apicultural Research
Volume52
Issue number1
DOIs
StatePublished - Apr 1 2013

Keywords

  • American foulbrood
  • BEEBOOK
  • Brood
  • COLOSS
  • Disease
  • Honey bee
  • Paenibacillus larvae
  • Pathogen
  • Technique

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    De Graaf, D. C., Alippi, A. M., Antúnez, K., Aronstein, K. A., Budge, G., De Koker, D., De Smet, L., Dingman, D. W., Evans, J. D., Foster, L. J., Fünfhaus, A., Garcia-Gonzalez, E., Gregorc, A., Human, H., Murray, K. D., Nguyen, B. K., Poppinga, L., Spivak, M., Van Engelsdorp, D., ... Genersch, E. (2013). Standard methods for American foulbrood research. Journal of Apicultural Research, 52(1). https://doi.org/10.3896/IBRA.1.52.1.11