Stage-specific embryonic antigen

Determining expression in canine glioblastoma, melanoma, and mammary cancer cells

Weiming Lin, Jaime Modiano, Daisuke Ito

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1- and SSEA-1+ cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalJournal of veterinary science
Volume18
Issue number1
DOIs
StatePublished - Mar 1 2017

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Stage-Specific Embryonic Antigens
CD15 Antigens
Glioblastoma
melanoma
breasts
Canidae
Melanoma
Breast Neoplasms
antigens
dogs
Cell Line
Hemangiosarcoma
Osteosarcoma
cell lines
neoplasm cells
hemangiosarcoma
Lymphoma
Neoplasms
osteosarcoma
Flow Cytometry

Keywords

  • Canine cancer
  • Flow cytometry
  • Magnetic-activated cell sorting
  • Stage-specific embryonic antigens

Cite this

Stage-specific embryonic antigen : Determining expression in canine glioblastoma, melanoma, and mammary cancer cells. / Lin, Weiming; Modiano, Jaime; Ito, Daisuke.

In: Journal of veterinary science, Vol. 18, No. 1, 01.03.2017, p. 101-104.

Research output: Contribution to journalArticle

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