Stage-specific embryonic antigen: Determining expression in canine glioblastoma, melanoma, and mammary cancer cells

Weiming Lin, Jaime F. Modiano, Daisuke Ito

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1- and SSEA-1+ cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalJournal of veterinary science
Volume18
Issue number1
DOIs
StatePublished - Mar 1 2017

Keywords

  • Canine cancer
  • Flow cytometry
  • Magnetic-activated cell sorting
  • Stage-specific embryonic antigens

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