Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system

Bethany A. Stahl, Robert Peuß, Brittnee McDole, Alexander Kenzior, James B. Jaggard, Karin Gaudenz, Jaya Krishnan, Suzanne E. McGaugh, Erik R. Duboue, Alex C. Keene, Nicolas Rohner

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Background: Astyanax mexicanus is a well-established fish model system for evolutionary and developmental biology research. These fish exist as surface forms that inhabit rivers and 30 different populations of cavefish. Despite important progress in the deployment of new technologies, deep mechanistic insights into the genetic basis of evolution, development, and behavior have been limited by a lack of transgenic lines commonly used in genetic model systems. Results: Here, we expand the toolkit of transgenesis by characterizing two novel stable transgenic lines that were generated using the highly efficient Tol2 system, commonly used to generate transgenic zebrafish. A stable transgenic line consisting of the zebrafish ubiquitin promoter expresses enhanced green fluorescent protein ubiquitously throughout development in a surface population of Astyanax. To define specific cell-types, a Cntnap2-mCherry construct labels lateral line mechanosensory neurons in zebrafish. Strikingly, both constructs appear to label the predicted cell types, suggesting many genetic tools and defined promoter regions in zebrafish are directly transferrable to cavefish. Conclusion: The lines provide proof-of-principle for the application of Tol2 transgenic technology in A. mexicanus. Expansion on these initial transgenic lines will provide a platform to address broadly important problems in the quest to bridge the genotype-phenotype gap.

Original languageEnglish (US)
Pages (from-to)679-687
Number of pages9
JournalDevelopmental Dynamics
Issue number8
StatePublished - Aug 2019

Bibliographical note

Funding Information:
Deutsche Forschungsgemeinschaft, Grant/ Award Number: PE 2807/1-1; Edward Mallinckrodt Foundation; NIH, Grant/Award Numbers: R01 GM1278872, R21 NS105071; Stowers Institute for Medical Research; NSF, Grant/Award Numbers: 1656574, 1754231

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.


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