Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system

Bethany A. Stahl, Robert Peuß, Brittnee McDole, Alexander Kenzior, James B. Jaggard, Karin Gaudenz, Jaya Krishnan, Suzanne E McGaugh, Erik R. Duboue, Alex C. Keene, Nicolas Rohner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Astyanax mexicanus is a well-established fish model system for evolutionary and developmental biology research. These fish exist as surface forms that inhabit rivers and 30 different populations of cavefish. Despite important progress in the deployment of new technologies, deep mechanistic insights into the genetic basis of evolution, development, and behavior have been limited by a lack of transgenic lines commonly used in genetic model systems. Results: Here, we expand the toolkit of transgenesis by characterizing two novel stable transgenic lines that were generated using the highly efficient Tol2 system, commonly used to generate transgenic zebrafish. A stable transgenic line consisting of the zebrafish ubiquitin promoter expresses enhanced green fluorescent protein ubiquitously throughout development in a surface population of Astyanax. To define specific cell-types, a Cntnap2-mCherry construct labels lateral line mechanosensory neurons in zebrafish. Strikingly, both constructs appear to label the predicted cell types, suggesting many genetic tools and defined promoter regions in zebrafish are directly transferrable to cavefish. Conclusion: The lines provide proof-of-principle for the application of Tol2 transgenic technology in A. mexicanus. Expansion on these initial transgenic lines will provide a platform to address broadly important problems in the quest to bridge the genotype-phenotype gap.

Original languageEnglish (US)
Pages (from-to)679-687
Number of pages9
JournalDevelopmental Dynamics
Volume248
Issue number8
DOIs
StatePublished - Jan 1 2019

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Transposases
Gene Transfer Techniques
Zebrafish
Fishes
Technology
Developmental Biology
Systems Biology
Molecular Evolution
Genetic Models
Ubiquitin
Genetic Promoter Regions
Rivers
Population
Genotype
Phenotype
Neurons
Research

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Stahl, B. A., Peuß, R., McDole, B., Kenzior, A., Jaggard, J. B., Gaudenz, K., ... Rohner, N. (2019). Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system. Developmental Dynamics, 248(8), 679-687. https://doi.org/10.1002/dvdy.32

Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system. / Stahl, Bethany A.; Peuß, Robert; McDole, Brittnee; Kenzior, Alexander; Jaggard, James B.; Gaudenz, Karin; Krishnan, Jaya; McGaugh, Suzanne E; Duboue, Erik R.; Keene, Alex C.; Rohner, Nicolas.

In: Developmental Dynamics, Vol. 248, No. 8, 01.01.2019, p. 679-687.

Research output: Contribution to journalArticle

Stahl, BA, Peuß, R, McDole, B, Kenzior, A, Jaggard, JB, Gaudenz, K, Krishnan, J, McGaugh, SE, Duboue, ER, Keene, AC & Rohner, N 2019, 'Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system', Developmental Dynamics, vol. 248, no. 8, pp. 679-687. https://doi.org/10.1002/dvdy.32
Stahl BA, Peuß R, McDole B, Kenzior A, Jaggard JB, Gaudenz K et al. Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system. Developmental Dynamics. 2019 Jan 1;248(8):679-687. https://doi.org/10.1002/dvdy.32
Stahl, Bethany A. ; Peuß, Robert ; McDole, Brittnee ; Kenzior, Alexander ; Jaggard, James B. ; Gaudenz, Karin ; Krishnan, Jaya ; McGaugh, Suzanne E ; Duboue, Erik R. ; Keene, Alex C. ; Rohner, Nicolas. / Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system. In: Developmental Dynamics. 2019 ; Vol. 248, No. 8. pp. 679-687.
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