Stability of Protein-Specific Hydration Shell on Crowding

Kuo Ying Huang, Carolyn N. Kingsley, Ryan Sheil, Chi Yuan Cheng, Jan C. Bierma, Kyle W. Roskamp, Domarin Khago, Rachel W. Martin, Songi Han

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

We demonstrate that the effect of protein crowding is critically dependent on the stability of the protein's hydration shell, which can dramatically vary between different proteins. In the human eye lens, γS-crystallin (γS-WT) forms a densely packed transparent hydrogel with a high refractive index, making it an ideal system for studying the effects of protein crowding. A single point mutation generates the cataract-related variant γS-G18V, dramatically altering the optical properties of the eye lens. This system offers an opportunity to explore fundamental questions regarding the effect of protein crowding, using γS-WT and γS-G18V: (i) how do the diffusion dynamics of hydration water change as a function of protein crowding?; and (ii) upon hydrogel formation of γS-WT, has a dynamic transition occurred generating a single population of hydration water, or do populations of bulk and hydration water coexist? Using localized spin probes, we separately probe the local translational diffusivity of both surface hydration and interstitial water of γS-WT and γS-G18V in solution. Surprisingly, we find that under the influence of hydrogel formation at highly crowded γS-WT concentrations up to 500 mg/mL, the protein hydration shell remains remarkably dynamic, slowing by less than a factor of 2, if at all, compared to that in dilute protein solutions of ∼5 mg/mL. Upon self-crowding, the population of this robust surface hydration water increases, while a significant bulk-like water population coexists even at ∼500 mg/mL protein concentrations. In contrast, surface water of γS-G18V irreversibly dehydrates with moderate concentration increases or subtle alterations to the solution conditions, demonstrating that the effect of protein crowding is highly dependent on the stability of the protein-specific hydration shell. The core function of γS-crystallin in the eye lens may be precisely its capacity to preserve a robust hydration shell, whose stability is abolished by a single G18V mutation.

Original languageEnglish (US)
Pages (from-to)5392-5402
Number of pages11
JournalJournal of the American Chemical Society
Volume138
Issue number16
DOIs
StatePublished - Apr 27 2016
Externally publishedYes

Bibliographical note

Funding Information:
S.H., K.H., and R.S. made up of the MRL Facilities supported by the NSF through the Materials Research Science and Engineering Centers under Grant DMR 1121053. S.H., K.H., and R.S. acknowledge the support of the IRG1 group of the MRL. Brendan Allison is acknowledged for his aid in researching the background literature relevant to our study as well as significant contribution to composing and editing this article. S.H., K.H., and R.S. acknowledge support from the 2011 NIH Director New Innovator Award and the Cluster of Excellence RESOLV (EXC 1069) funded by the Deutsche Forschungsgemeinschaft. R.W.M, C.N.K., J.C.B, K.W.R., and D.K. acknowledge support from NIH R01EY021514 to R.W.M.

Publisher Copyright:
© 2016 American Chemical Society.

Fingerprint

Dive into the research topics of 'Stability of Protein-Specific Hydration Shell on Crowding'. Together they form a unique fingerprint.

Cite this