Stability of Polysome-Associated Polyadenylated RNA from Soybean Suspension Culture Cells

Carolyn D. Silflow, Joe L. Key, Joe L. Key2

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The half-life of polysome-associated, poly(A)-RNA in exponentially growing soybean (Glycine max) suspension culture cells was determined with pulse-chase experiments. Based on a best fit from a computer analysis of the data, two decay components for poly(A)-RNA were found. One component had a half-life of approximately 0.6 h, while the other had a half-life of about 30 h, similar to the doubling time of the cultures. At the beginning of the chase period, the short-lived component represented approximately 90% of the total poly(A)-RNA in the polysomes. This percentage decreased with time so that, under steady-state conditions, the long-lived component probably represented the majority of poly(A)-RNA.

Original languageEnglish (US)
Pages (from-to)1013-1018
Number of pages6
JournalBiochemistry
Volume18
Issue number6
DOIs
StatePublished - Jan 1 1979

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