Abstract
Differential scanning calorimetry, intrinsic fluorescence and SDS-polyacrylamide-gel-electrophoresis have been applied for the study of the low molecular mass cytotoxin from the sea anemone, Radianthus macrodactylus when in solution and in complexes with phospholipids and erythrocyte membranes. The thermodynamic parameters of toxin denaturation in solution have been obtained. It is shown that one molecule of this toxin withdraws 10±2 dipalmitoylphosphatidylcholine molecules through cooperative transition. The effect of toxin on dog erythrocytes causes a change in the polypeptide contents of erythrocyte ghosts.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 331-340 |
| Number of pages | 10 |
| Journal | Biochemistry and Molecular Biology International |
| Volume | 32 |
| Issue number | 2 |
| State | Published - 1994 |
| Externally published | Yes |