Stability of a low molecular mass cytotoxin from the sea anemone Radianthus macrodactylus and membrane toxin interactions

G. Zhadan, S. Kuznetsova, O. Opalikova, M. Monastyrnaya, T. Zykova, V. Emelyanenko, E. Villar, V. Shnyrov

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Abstract

Differential scanning calorimetry, intrinsic fluorescence and SDS-polyacrylamide-gel-electrophoresis have been applied for the study of the low molecular mass cytotoxin from the sea anemone, Radianthus macrodactylus when in solution and in complexes with phospholipids and erythrocyte membranes. The thermodynamic parameters of toxin denaturation in solution have been obtained. It is shown that one molecule of this toxin withdraws 10±2 dipalmitoylphosphatidylcholine molecules through cooperative transition. The effect of toxin on dog erythrocytes causes a change in the polypeptide contents of erythrocyte ghosts.

Original languageEnglish (US)
Pages (from-to)331-340
Number of pages10
JournalBiochemistry and Molecular Biology International
Volume32
Issue number2
StatePublished - Jan 1 1994

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    Zhadan, G., Kuznetsova, S., Opalikova, O., Monastyrnaya, M., Zykova, T., Emelyanenko, V., Villar, E., & Shnyrov, V. (1994). Stability of a low molecular mass cytotoxin from the sea anemone Radianthus macrodactylus and membrane toxin interactions. Biochemistry and Molecular Biology International, 32(2), 331-340.