Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus

A. D. Krikorian, H. Irizarry, R. Goenaga, Mary E. Scott, B. E.L. Lockhart

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Field evaluation of plants produced in vitro initiated from a phenotypically distinct, apparently healthy dwarf 'French-type' plantain clone 'Dwarf Superplatano' (Musa 'AAB') was carried out at three sites in Puerto Rico. The use of the floral axis tip from a 'mother plant' versus vegetative apices from lateral buds of the same plant as a source of the primary explant was compared and contrasted. Material from floral axis tip consistently showed high phenotypic uniformly whereas materials from vegetative apices of 'sword' suckers were less so. 'Virus-like symptoms' that became apparent in much of the material just before flowering (shooting stage) were determined to be due to the badnavirus banana streak virus (BSV), a dsDNA pararetrovirus. The 'good news' is that a primary explant taken from the floral axis tip was quicker in its initial response to yield a multiplication system in vitro, and produced significantly fewer virus-infected plants, ca. 5%. By contrast, primary explants obtained from the vegetative sucker-derived apices were later in their production of initial buds, and produced many more virus-infected plants, an average of 32%. Comparison of vegetative-apex-derived plants and floral axis tip-derived plants disclosed no evidence that apices from vegetative suckers or floral stem tips gave rise to genetic off-types due to mutations were brought about by the in vitro process per se. Phenotypic differences were due to virus infestation. The 'bad news' is that this dwarf plantain clone of considerable interest and potential in Puerto Rico can show very severe BSV symptoms. The occurrence of BSV infection in tissue culture-derived plants may be related to the presence of viral sequences integrated into the host genome, in which case there is no apparent strategy to rid the clone of this 'virus'.

Original languageEnglish (US)
Pages (from-to)159-177
Number of pages19
JournalScientia Horticulturae
Volume81
Issue number2
DOIs
StatePublished - Jun 1 1999

Keywords

  • Banana streak virus (BSV)
  • Clonal multiplication
  • Musa
  • Phenotype
  • Plantains
  • Somaclonal variation
  • Tissue culture

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