Stability and tick transmission phenotype of gfp-transformed Anaplasma marginale through a complete in vivo infection cycle

Susan M. Noh, Massaro W. Ueti, Guy H. Palmer, Ulrike G. Munderloh, Roderick F. Felsheim, Kelly A. Brayton

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We tested the stability and tick transmission phenotype of transformed Anaplasma marginale through a complete in vivo infection cycle. Similar to the wild type, the gfp-transformed A. marginale strain established infection in cattle, a natural reservoir host, and persisted in immune competent animals. The tick infection rates for the transformed A. marginale and the wild type were the same. However, there were significantly lower levels of the transformed A. marginale than of the wild type in the tick. Despite the lower levels of replication, ticks transmitted the transformant. Transformants can serve as valuable tools to dissect the molecular requirements of tick colonization and pathogen transmission.

Original languageEnglish (US)
Pages (from-to)330-334
Number of pages5
JournalApplied and environmental microbiology
Volume77
Issue number1
DOIs
StatePublished - Jan 2011

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