Abstract
Human SRC encodes the non-receptor tyrosine kinase pp60c-Src, which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 367-371 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 487 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 5 2001 |
Bibliographical note
Funding Information:We thank Dr. D. Anderson for helping to establish in our laboratory several of the techniques used in this report. This work was supported with grants from the Health Services Utilization and Research Commission of Saskatchewan and the Medical Research Council of Canada to K.B. S.D. was funded with a University of Saskatchewan College of Medicine Graduate Research Scholarship.
Keywords
- Colon cancer
- Oncogene activation
- Transcription
- c-Src
