SRC transcriptional activation in a subset of human colon cancer cell lines

Scott Dehm, Mary Anne Senger, Keith Bonham

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Human SRC encodes the non-receptor tyrosine kinase pp60c-Src, which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.

Original languageEnglish (US)
Pages (from-to)367-371
Number of pages5
JournalFEBS Letters
Volume487
Issue number3
DOIs
StatePublished - Jan 5 2001

Keywords

  • Colon cancer
  • Oncogene activation
  • Transcription
  • c-Src

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