Glyphosate is one of the most widely used nonselective herbicides. The nearly ubiquitous use of glyphosate-resistant soybean [Glycine max (L.) Merr.], corn (Zea mays L.), sugar beet (Beta vulgaris L.), and/or canola (Brassica napus L.) cultivars in the Northern Great Plains can, unfortunately, result in off-target movement to sensitive crops. Off-target movement generally exposes a sensitive crop like spring wheat (Triticum aestivum L.) to rates ranging from 1 to 10% of the applied rate. The objective of this study was to measure the effect of simulated glyphosate drift at a concentration of 0.07 lb acid equivalent/acre on spring wheat crop injury, crop residue, and yield. At 28 d after treatment (DAT), crop injury varied from none to 67.5%. Across years, the application at Zadoks GS17 resulted in the most crop injury, with symptoms typical of glyphosate injury as the crop was severely stunted and yellow at 28 DAT, resulting in yield losses between 82 and 96% of the untreated control. Glyphosate residue could be detected at 7 DAT for all treatments, but the number of false negatives increased to over half at 21 DAT. No clear quantitative relationship could be discerned from the amount of glyphosate residue detected and the application timing.