Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled γ-phosphate-linked GTP analogs

Keyphrases

• Phosphate 100%
• Low Molecular Weight 100%
• Fluorescent Labeling 100%
• GTPase 100%
• Nucleotide Exchange 100%
• GTP Analogs 100%
• Protein Binding 40%
• Cdc42 40%
• Mg2+ 40%
• Fluorescence Enhancement 40%
• BODIPY Fluorophores 40%
• Fluorophore 20%
• Exchange Rate 20%
• Bound States 20%
• Guanine 20%
• Signaling Molecules 20%
• State-resolved 20%
• Time Monitoring 20%
• Steady State 20%
• Binding Affinity 20%
• Diphosphate 20%
• High Signal-to-noise Ratio 20%
• Optimization Criterion 20%
• Rac1 20%
• Guanosine 20%
• Guanosine nucleotides 20%
• RhoA 20%
• Guanosine Triphosphate 20%
• Synthetic Oligonucleotides 20%
• Visible Spectrum 20%
• Linker Length 20%
• Environmental Sensitivity 20%
• Guanine nucleotide Exchange Factor 20%
• Active Site Geometry 20%
• PAK1 20%
• Triphosphate Analogues 20%
• Nucleotide Hydrolysis 20%
• Steady Time 20%
• Rapid Loading 20%
• Dye Fluorescence 20%
• Nucleotide Exchange Factor 20%
• Time-resolved Fluorescence Spectroscopy 20%
• BODIPY Dyes 20%
• Aliphatic Linkers 20%
• BODIPY Analogs 20%

Neuroscience

• Guanosine Triphosphate 100%
• Guanosine 100%
• Fluorescent Dye 50%
• Spectroscopy 50%
• GTPase 50%
• Signal-to-Noise Ratio 50%
• Guanine 50%
• Binding Affinity 50%
• Protein Binding 50%
• Pyrophosphate 50%
• Ethylenediaminetetraacetic Acid 50%
• Guanine Nucleotide Exchange Factor 50%
• P21 Activated Kinase 50%

Biochemistry, Genetics and Molecular Biology

• Molecular Weight 100%
• Guanosine Triphosphate 100%
• Nucleotide 100%
• Guanosine 33%
• Active Site 16%
• Binding Protein 16%
• Enzymatic Hydrolysis 16%
• P21-Activated Kinases 16%
• Nucleotide Exchange Factor 16%
• Steady State 16%
• Binding Affinity 16%
• Signal Noise Ratio 16%
• GTPase 16%
• Pyrophosphate 16%
• Guanine Nucleotide Exchange Factor 16%
• Fluorescence Spectroscopy 16%
• Guanine 16%