Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain

Alicia Aranaz, Beatriz Romero, Natalia Montero, Julio Álvarez, Javier Bezos, Lucía De Juan, Ana Mateos, Lucas Domínguez

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.

Original languageEnglish (US)
Pages (from-to)5388-5391
Number of pages4
JournalJournal of clinical microbiology
Volume42
Issue number11
DOIs
StatePublished - Nov 2004
Externally publishedYes

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