Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci

David A. Mills, Larry L. Mckay, Gary M. Dunny

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

Analysis of a region involved in the conjugative transfer of the lactococcal conjugative element pRS01 has revealed a bacterial group II intron. Splicing of this lactococcal intron (designated Ll.ltrB) in vivo resulted in the ligation of two exon messages (ltrBE1 and ltrBE2) which encoded a putative conjugative relaxase essential for the transfer of pRS01. Like many group II introns, the Ll.ltrB intron possessed an open reading frame (ltrA) with homology to reverse transcriptases. Remarkably, sequence analysis of ltrA suggested a greater similarity to open reading frames encoded by eukaryotic mitochondrial group II introns than to those identified to date from other bacteria. Several insertional mutations within ltrA resulted in plasmids exhibiting a conjugative transfer-deficient phenotype. These results provide the first direct evidence for splicing of a prokaryotic group II intron in vivo and suggest that conjugative transfer is a mechanism for group II intron dissemination in bacteria.

Original languageEnglish (US)
Pages (from-to)3531-3538
Number of pages8
JournalJournal of bacteriology
Volume178
Issue number12
DOIs
StatePublished - Jun 1996

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