Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

Bradley J. Segura; Weizhen Zhang; Liqun Xiao; Craig D. Logsdon; Michael W. Mulholland

doi:10.1016/j.molbrainres.2004.10.016

Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

Bradley J. Segura
, Weizhen Zhang
, Liqun Xiao
, Craig D. Logsdon
, Michael W. Mulholland

Surgery

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c-fos activation by S1P (100 μM-187±6% vs. 411±27%) and lysophosphatidic acid (LPA; 100 μM-90±34% vs. 188±41%), but not by sphingosylphosphorylcholine (SPC; 100 μM-390±47% vs. 420±44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.

Original language	English (US)
Pages (from-to)	325-328
Number of pages	4
Journal	Molecular Brain Research
Volume	133
Issue number	2
DOIs	https://doi.org/10.1016/j.molbrainres.2004.10.016
State	Published - Feb 18 2005

Bibliographical note

Funding Information:
This research was supported by the National Institutes of Health (DK41204 and accompanying Minority Supplemental Grant, and Systems and Integrative Biology training grant GM08322-11) and by a pre-doctoral fellowship from the Horace Rackham School of Graduate Studies, University of Michigan.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

C-fos
Early response gene
Glia
Lysophosphatidic acid
Sphingosine-1-phosphate
Sphingosylphosphorylcholine

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10.1016/j.molbrainres.2004.10.016

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title = "Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells",

abstract = "Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c-fos activation by S1P (100 μM-187±6\% vs. 411±27\%) and lysophosphatidic acid (LPA; 100 μM-90±34\% vs. 188±41\%), but not by sphingosylphosphorylcholine (SPC; 100 μM-390±47\% vs. 420±44\%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.",

keywords = "C-fos, Early response gene, Glia, Lysophosphatidic acid, Sphingosine-1-phosphate, Sphingosylphosphorylcholine",

author = "Segura, \{Bradley J.\} and Weizhen Zhang and Liqun Xiao and Logsdon, \{Craig D.\} and Mulholland, \{Michael W.\}",

note = "Funding Information: This research was supported by the National Institutes of Health (DK41204 and accompanying Minority Supplemental Grant, and Systems and Integrative Biology training grant GM08322-11) and by a pre-doctoral fellowship from the Horace Rackham School of Graduate Studies, University of Michigan.",

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month = feb,

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doi = "10.1016/j.molbrainres.2004.10.016",

language = "English (US)",

volume = "133",

pages = "325--328",

journal = "Molecular Brain Research",

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TY - JOUR

T1 - Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

AU - Segura, Bradley J.

AU - Zhang, Weizhen

AU - Xiao, Liqun

AU - Logsdon, Craig D.

AU - Mulholland, Michael W.

N1 - Funding Information: This research was supported by the National Institutes of Health (DK41204 and accompanying Minority Supplemental Grant, and Systems and Integrative Biology training grant GM08322-11) and by a pre-doctoral fellowship from the Horace Rackham School of Graduate Studies, University of Michigan.

PY - 2005/2/18

Y1 - 2005/2/18

N2 - Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c-fos activation by S1P (100 μM-187±6% vs. 411±27%) and lysophosphatidic acid (LPA; 100 μM-90±34% vs. 188±41%), but not by sphingosylphosphorylcholine (SPC; 100 μM-390±47% vs. 420±44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.

AB - Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c-fos activation by S1P (100 μM-187±6% vs. 411±27%) and lysophosphatidic acid (LPA; 100 μM-90±34% vs. 188±41%), but not by sphingosylphosphorylcholine (SPC; 100 μM-390±47% vs. 420±44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.

KW - C-fos

KW - Early response gene

KW - Glia

KW - Lysophosphatidic acid

KW - Sphingosine-1-phosphate

KW - Sphingosylphosphorylcholine

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UR - https://www.scopus.com/pages/publications/13844289243#tab=citedBy

U2 - 10.1016/j.molbrainres.2004.10.016

DO - 10.1016/j.molbrainres.2004.10.016

M3 - Article

C2 - 15710251

AN - SCOPUS:13844289243

SN - 0169-328X

VL - 133

SP - 325

EP - 328

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 2

ER -

Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

Abstract

Bibliographical note

UN SDGs

Keywords

Publisher link

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