Chemically modified silica gels were prepared under various conditions with γ-glycidoxypropyltrimethoxysilane in order to investigate the effect of various methods of hydrolyzing the terminal epoxide group. The silylated substrates were then examined by 13C solid-state NMR with cross-polarization and magic angle spinning. NMR results were correlated with infrared results obtained by diffuse reflectance Fourier transform infrared spectroscopy. By reaction with a primary alkyl amine the percentage of unopened epoxide may be determined by Fourier transform infrared spectroscopy. Infrared data indicate that as little as 10% epoxide remaining after attempted hydrolysis can influence chromatographic capacity factors.
Bibliographical noteFunding Information:
This researchw as supported in part by Research Grant CHE-87 12457f rom the National Science Foundation. T.M.W., W.S. and P.W.C. acknowledge the Center for Bioprocess Technology of the University of Minnesota. G.R.B. and D.E.L. acknowledge the Colorado State University NMR Center, funded by National Science
Foundation Regional Instrumentation Center Grant CHE-8616437. The Nicolet 60SX FTIR spectrometer was purchased in part from a grant from the National Science Foundation (CHE-8317079).