Specific detection of avian pneumovirus (APV) US isolates by RT-PCR

H. J. Shin; G. Rajashekara; F. F. Jirjis; D. P. Shaw; S. M. Goyal; D. A. Halvorson; K. V. Nagaraja

doi:10.1007/s007050070123

Specific detection of avian pneumovirus (APV) US isolates by RT-PCR

H. J. Shin, G. Rajashekara, F. F. Jirjis, D. P. Shaw, S. M. Goyal, D. A. Halvorson, K. V. Nagaraja

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Abstract

This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV). Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as tow as 8 x 10^-5 TCID₅₀ (0.0323 μg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from turkeys in Minnesota.

Original language	English (US)
Pages (from-to)	1239-1246
Number of pages	8
Journal	Archives of Virology
Volume	145
Issue number	6
DOIs	https://doi.org/10.1007/s007050070123
State	Published - 2000

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title = "Specific detection of avian pneumovirus (APV) US isolates by RT-PCR",

abstract = "This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV). Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as tow as 8 x 10-5 TCID50 (0.0323 μg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from turkeys in Minnesota.",

author = "Shin, {H. J.} and G. Rajashekara and Jirjis, {F. F.} and Shaw, {D. P.} and Goyal, {S. M.} and Halvorson, {D. A.} and Nagaraja, {K. V.}",

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T1 - Specific detection of avian pneumovirus (APV) US isolates by RT-PCR

AU - Shin, H. J.

AU - Rajashekara, G.

AU - Jirjis, F. F.

AU - Shaw, D. P.

AU - Goyal, S. M.

AU - Halvorson, D. A.

AU - Nagaraja, K. V.

PY - 2000

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N2 - This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV). Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as tow as 8 x 10-5 TCID50 (0.0323 μg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from turkeys in Minnesota.

AB - This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV). Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as tow as 8 x 10-5 TCID50 (0.0323 μg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from turkeys in Minnesota.

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JO - Archives of Virology

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Specific detection of avian pneumovirus (APV) US isolates by RT-PCR

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