TY - JOUR
T1 - Species differentiation by internally transcribed spacer PCR and HhaI digestion of fluconazole-resistant Candida krusei, Candida inconspicua, and Candida norvegensis strains
AU - Nho, Seonghun
AU - Anderson, Michael J.
AU - Moore, Caroline B.
AU - Denning, David W.
PY - 1997/4
Y1 - 1997/4
N2 - PCR amplification of the regions containing the internally transcribed spacers and 5.8S rRNA gene of Candida krusei, C. inconspicua, and C. norvegensis yielded fragments of 510, 460, and 500 bp, respectively. HhaI digestion of these fragments yielded species-specific bands. Random amplification of polymorphic DNA with primer R108 showed interspecific discriminatory band patterns. Susceptibilities to fluconazole and amphotericin B were determined.
AB - PCR amplification of the regions containing the internally transcribed spacers and 5.8S rRNA gene of Candida krusei, C. inconspicua, and C. norvegensis yielded fragments of 510, 460, and 500 bp, respectively. HhaI digestion of these fragments yielded species-specific bands. Random amplification of polymorphic DNA with primer R108 showed interspecific discriminatory band patterns. Susceptibilities to fluconazole and amphotericin B were determined.
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U2 - 10.1128/jcm.35.4.1036-1039.1997
DO - 10.1128/jcm.35.4.1036-1039.1997
M3 - Article
C2 - 9157129
AN - SCOPUS:0030910408
SN - 0095-1137
VL - 35
SP - 1036
EP - 1039
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 4
ER -