Spatial regulation of wingless morphogen distribution and signaling by Dally-like protein

Catherine Kirkpatrick, Brian D. Dimitroff, Jaime M. Rawson, Scott B. Selleck

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Wingless (Wg) is a morphogen required for the patterning of many Drosophila tissues. Several lines of evidence implicate heparan sulfate-modified proteoglycans (HSPGs) such as Dally-like protein (Dlp) in the control of Wg distribution and signaling. We show that dlp is required to limit Wg levels in the matrix, contrary to the expectation from overexpression studies. dlp mutants show ectopic activation of Wg signaling at the presumptive wing margin and a local increase in extracellular Wg levels. dlp somatic cell clones disrupt the gradient of extracellular Wg, producing ectopic activation of high threshold Wg targets but reducing the expression of lower threshold Wg targets where Wg is limiting. Notum encodes a secreted protein that also limits Wg distribution, and genetic interaction studies show that dlp and Notum cooperate to restrict Wg signaling. These findings suggest that modification of an HSPG by a secreted hydrolase can control morphogen levels in the matrix.

Original languageEnglish (US)
Pages (from-to)513-523
Number of pages11
JournalDevelopmental Cell
Volume7
Issue number4
DOIs
StatePublished - Oct 1 2004

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Heparan Sulfate Proteoglycans
Heparitin Sulfate
Proteoglycans
Clone cells
Hydrolases
Chemical activation
Drosophila
Proteins
Clone Cells
Level control
Tissue

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Spatial regulation of wingless morphogen distribution and signaling by Dally-like protein. / Kirkpatrick, Catherine; Dimitroff, Brian D.; Rawson, Jaime M.; Selleck, Scott B.

In: Developmental Cell, Vol. 7, No. 4, 01.10.2004, p. 513-523.

Research output: Contribution to journalArticle

Kirkpatrick, Catherine ; Dimitroff, Brian D. ; Rawson, Jaime M. ; Selleck, Scott B. / Spatial regulation of wingless morphogen distribution and signaling by Dally-like protein. In: Developmental Cell. 2004 ; Vol. 7, No. 4. pp. 513-523.
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