Spatial and temporal variation in nitrogen fixation and its importance to phytoplankton in phosphorus-rich lakes

Nicole M. Hayes, Alain Patoine, Heather A. Haig, Gavin L. Simpson, Vanessa J. Swarbrick, Emma Wiik, Peter R. Leavitt

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Limnological theory posits that phosphorus (P) limits primary production in freshwater lakes, in part because fixation of atmospheric nitrogen (N 2 ) can compensate for limitations in nitrogen (N) supply to phytoplankton. However, quantitative estimates of the degree to which N 2 fixation satisfies planktonic N demand are rare. Here we used biweekly sampling during summer in seven lakes over 2 decades to estimate both planktonic N 2 fixation and phytoplankton N demand. We further assessed the ability of biologically fixed N to satisfy N needs of primary producers in productive hardwater lakes. Phytoplankton N requirements, derived from estimates of phytoplankton productivity and N content, were moderately synchronous (S = 0.41) among lakes (ca. 0.1–9.2 mg N m –3  hr –1 ). In contrast, rates of N 2 fixation determined using isotopic natural abundance method (NAM; 0.002–3.2 mg N m –3  hr –1 ), or heterocyte-based calculations (0.10–1.78 mg N m –3  hr –1 ), varied asynchronously (S NAM  = –0.03 and S Heterocyte  = –0.11) among basins, accounted for a median of 3.5% (mean 11.3% ± 21.6) of phytoplankton demand, and were correlated to the abundance of Nostocales cyanobacteria when analysed using generalised additive models. Overall, the total mass of fixed N accounted for a median of only 3.0% of the spring standing stock of total dissolved N in study lakes (mean 7.5 ± 12.1%), with higher relative importance of fixed N in highly productive downstream lakes. Thus, while fixed N helps sustain primary productivity, particularly in years with high rates of N 2 -fixation, it does not appear to eliminate N limitation of phytoplankton growth in these P-rich hardwater lakes.

Original languageEnglish (US)
Pages (from-to)269-283
Number of pages15
JournalFreshwater Biology
Volume64
Issue number2
DOIs
StatePublished - Feb 1 2019

Bibliographical note

Publisher Copyright:
© 2018 The Authors. Freshwater Biology Published by John Wiley & Sons Ltd.

Keywords

  • cyanobacteria
  • nitrogen demand
  • nutrient limitation
  • stable isotopes
  • δ N

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