Sp1 and CREB regulate basal transcription of the human SNF2L gene

Yu Xia, Baichun Jiang, Yongxin Zou, Guimin Gao, Linshan Shang, Bingxi Chen, Qiji Liu, Yaoqin Gong

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Imitation Switch (ISWI) is a member of the SWI2/SNF2 superfamily of ATP-dependent chromatin remodelers, which are involved in multiple nuclear functions, including transcriptional regulation, replication, and chromatin assembly. Mammalian genomes encode two ISWI orthologs, SNF2H and SNF2L. In order to clarify the molecular mechanisms governing the expression of human SNF2L gene, we functionally examined the transcriptional regulation of human SNF2L promoter. Reporter gene assays demonstrated that the minimal SNF2L promoter was located between positions -152 to -86 relative to the transcription start site. In this region we have identified a cAMP-response element (CRE) located at -99 to -92 and a Sp1-binding site at -145 to -135 that play a critical role in regulating basal activity of human SNF2L gene, which were proven by deletion and mutation of specific binding sites, EMSA, and down-regulating Sp1 and CREB via RNAi. This study provides the first insight into the mechanisms that control basal expression of human SNF2L gene.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume368
Issue number2
DOIs
StatePublished - Apr 4 2008
Externally publishedYes

Keywords

  • Basal promoter
  • CRE
  • ISWI
  • SNF2L
  • Sp1

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