Soy isoflavones enhance β-defensin synthesis and secretion in endometrial epithelial cells with exposure to TLR3 agonist polyinosinic-polycytidylic acid

Yotesawee Srisomboon, Sutthasinee Poonyachoti, Chatsri Deachapunya

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Problem: β-defensins are important innate chemical barriers that protect the endometrium from pathogen invasion. The effects of soy isoflavones, genistein and daidzein, on the expression and secretion of porcine β-defensins (PBD) in endometrial epithelial cells were investigated under normal or poly I:C-stimulated conditions. Method of study: Primary cultured porcine endometrial epithelial (PE) cells were pretreated with genistein or daidzein followed by poly I:C inoculation. During treatment, the culture media were analyzed for PBD 1-4 secretion by ELISA and the total RNA for PBD gene expression by quantitative RT-PCR. Results: Porcine endometrial epithelial cells constitutively expressed PBD 1-4 and secreted PBD-1, PBD-2, and PBD-4. Genistein and daidzein enhanced PBD-2 expression and PBD-2 and PBD-3 secretion. These compounds also potentiated PBD-2 and PBD-3 expression and secretion which were upregulated by poly I:C. Conclusion: Soy isoflavones, genistein and daidzein, could be potentially used for promoting the innate host defense of endometrium against infection.

Original languageEnglish (US)
Article numbere12694
JournalAmerican Journal of Reproductive Immunology
Volume78
Issue number4
DOIs
StatePublished - 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Keywords

  • antimicrobial peptide
  • daidzein
  • endometrium
  • genistein
  • innate immunity

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