Sox7 Regulates Lineage Decisions in Cardiovascular Progenitor Cells

Michelle J. Doyle, Alessandro Magli, Nima Estharabadi, Danielle Amundsen, Lauren J. Mills, Cindy M. Martin

Research output: Contribution to journalArticle

Abstract

Specification of the mesodermal lineages requires a complex set of morphogenetic events orchestrated by interconnected signaling pathways and gene regulatory networks. The transcription factor Sox7 has critical functions in differentiation of multiple mesodermal lineages, including cardiac, endothelial, and hematopoietic. Using a doxycycline-inducible mouse embryonic stem cell line, we have previously shown that expression of Sox7 in cardiovascular progenitor cells promotes expansion of endothelial progenitor cells (EPCs). In this study, we show that the ability of Sox7 to promote endothelial cell fate occurs at the expense of the cardiac lineage. Using ChIP-Seq coupled with ATAC-Seq we identify downstream target genes of Sox7 in cardiovascular progenitor cells and by integrating these data with transcriptomic analyses, we define Sox7-dependent gene programs specific to cardiac and EPCs. Furthermore, we demonstrate a protein-protein interaction between SOX7 and GATA4 and provide evidence that SOX7 interferes with the transcriptional activity of GATA4 on cardiac genes. In addition, we show that Sox7 modulates WNT and BMP signaling during cardiovascular differentiation. Our data represent the first genome-wide analysis of Sox7 function and reveal a critical role for Sox7 in regulating signaling pathways that affect cardiovascular progenitor cell differentiation.

Original languageEnglish (US)
Pages (from-to)1089-1103
Number of pages15
JournalStem Cells and Development
Volume28
Issue number16
DOIs
StatePublished - Aug 15 2019

Keywords

  • Sox7
  • cardiovascular progenitor cells
  • transcriptional regulation

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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