Abstract
Identifying the resistant genotypes is one of the vital strategies to control Fusarium stalk rot disease in maize. Fifty accessions of maize germplasm were evaluated for resistance to stalk rot caused by Fusarium verticillioides at the Maize and Millet Research Institute, Yousafwala, Pakistan, during the spring and autumn of 2010, and their genetic variations were also studied at the molecular level to avoid environmental effects in the Department of Medicinal Chemistry, University of Minnesota, USA. Disease severity was calculated in the field using a severity scale (SS) and direct estimation of stalk discoloration (SD) by artificial inoculation method. Both SS and SD results were highly significant (P < 0.01) in both seasons and maize accessions significantly differed in SS (P < 0.01) and SD (P < 0.01). Disease assessments based on SS and SD were significantly correlated (r = 0.983, 0.974; P < 0.01) in spring and autumn, respectively. Two genotypes, EL7 and Y11, showed highly resistant response in both growing seasons. Variations among the lines were also exploited by using 14 simple sequence repeat primer sets. A total of 535 alleles were amplified with an average of 10.7 alleles per genotype. The highest number of alleles per locus was seen with the xp-umc1186 primer, exhibiting 128 alleles with an average of 2.5 alleles per genotype. Resistance genotypes can be utilized in hybridization programs for the improvement of local high-yielding varieties.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 503-513 |
| Number of pages | 11 |
| Journal | Turkish Journal of Agriculture and Forestry |
| Volume | 39 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2015 |
Bibliographical note
Funding Information:This research was funded by University Grant Commission (UGC), Nepal (Award No. 73/74/S & T-06). The authors are grateful to ICRE-UY, Japan for providing lab facilities and Ramesh Raj Pant for his support in statistical discussions. The authors would like to acknowledge the support of Science and Technology Research Partnership for Sustainable Development (SATREPS), Japan International Co-operation Agency (JICA), Japan Science and Technology Agency (JST), and Grants-in-Aid for Scientific Research (KAKENHI No. 18K11617).
Funding Information:
Funding: This research was funded by University Grant Commission (UGC), Nepal (Award No. 73/74/S & T-06).
Funding Information:
Acknowledgments: The authors are grateful to ICRE-UY, Japan for providing lab facilities and Ramesh Raj Pant for his support in statistical discussions. The authors would like to acknowledge the support of Science and Technology Research Partnership for Sustainable Development (SATREPS), Japan International Co-operation Agency (JICA), Japan Science and Technology Agency (JST), and Grants-in-Aid for Scientific Research (KAKENHI No. 18K11617).
Publisher Copyright:
© 2015 TÜBİTAK.
Keywords
- Alleles
- Fusarium stalk rot
- Maize
- Resistance