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Solution NMR structures of pyrenophora tritici-repentis toxb and its inactive homolog reveal potential determinants of toxin activity

  • Afua Nyarko
  • , Kiran K. Singarapu
  • , Melania Figueroa
  • , Viola A. Manning
  • , Iovanna Pandelova
  • , Thomas J. Wolpert
  • , Lynda M. Ciuffetti
  • , Elisar Barbar

Research output: Contribution to journalArticlepeer-review

Abstract

Background: ToxB is a proteinaceous toxin but its homolog toxb has no toxic activity.

Results: Both adopt a β-sandwich fold stabilized by two disulfide bonds but differ in the dynamics of one sandwich half.

Conclusion: Toxicity is correlated with decreased compactness, increased flexibility, and polymorphism in an active site loop.

Significance: ToxB activity depends on interplay between internal dynamics and interactions with putative targets.

Original languageEnglish (US)
Pages (from-to)25946-25956
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number37
DOIs
StatePublished - Sep 12 2014

Bibliographical note

Publisher Copyright:
© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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