Solubilization of membrane bound opiate receptor from rat brain

Tae Mook Cho; Chiyuki Yamato; Jung Sook Cho; Horace H. Loh

doi:10.1016/0024-3205(81)90723-2

Solubilization of membrane bound opiate receptor from rat brain

Tae Mook Cho, Chiyuki Yamato, Jung Sook Cho, Horace H. Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Sonication of rat brain membranes for 9 minutes solubilized 35% of their stereospecific opiate binding activity; a second 9 minute sonication of the insoluble residue released an additional 21% of the original binding. The opiate binding properties of the solubilized material were highly similar to those of membrane bound receptor by a number of criteria, including affinity, effect of sodium, and the IC₅₀ of unlabeled opiates in displacing ³H-etorphine binding. Moreover, storage of the solubilized receptor fraction for two weeks at -20°C did not significantly change the receptor binding. Sonication thus appears to be a useful first step in purifying the opiate receptor.

Original language	English (US)
Pages (from-to)	2651-2657
Number of pages	7
Journal	Life Sciences
Volume	28
Issue number	23
DOIs	https://doi.org/10.1016/0024-3205(81)90723-2
State	Published - Jun 8 1981

Bibliographical note

Funding Information:
This work was supported in part by Public Health Service Grant DA-01583. H.H.L. is a recipient of NIDA Research Career Development Award, DA-70554. The authors thank Dr. Andrew Smith for preparation of this manuscript.

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title = "Solubilization of membrane bound opiate receptor from rat brain",

abstract = "Sonication of rat brain membranes for 9 minutes solubilized 35% of their stereospecific opiate binding activity; a second 9 minute sonication of the insoluble residue released an additional 21% of the original binding. The opiate binding properties of the solubilized material were highly similar to those of membrane bound receptor by a number of criteria, including affinity, effect of sodium, and the IC50 of unlabeled opiates in displacing 3H-etorphine binding. Moreover, storage of the solubilized receptor fraction for two weeks at -20°C did not significantly change the receptor binding. Sonication thus appears to be a useful first step in purifying the opiate receptor.",

author = "Cho, {Tae Mook} and Chiyuki Yamato and Cho, {Jung Sook} and Loh, {Horace H.}",

note = "Funding Information: This work was supported in part by Public Health Service Grant DA-01583. H.H.L. is a recipient of NIDA Research Career Development Award, DA-70554. The authors thank Dr. Andrew Smith for preparation of this manuscript.",

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AU - Cho, Tae Mook

AU - Yamato, Chiyuki

AU - Cho, Jung Sook

AU - Loh, Horace H.

N1 - Funding Information: This work was supported in part by Public Health Service Grant DA-01583. H.H.L. is a recipient of NIDA Research Career Development Award, DA-70554. The authors thank Dr. Andrew Smith for preparation of this manuscript.

PY - 1981/6/8

Y1 - 1981/6/8

N2 - Sonication of rat brain membranes for 9 minutes solubilized 35% of their stereospecific opiate binding activity; a second 9 minute sonication of the insoluble residue released an additional 21% of the original binding. The opiate binding properties of the solubilized material were highly similar to those of membrane bound receptor by a number of criteria, including affinity, effect of sodium, and the IC50 of unlabeled opiates in displacing 3H-etorphine binding. Moreover, storage of the solubilized receptor fraction for two weeks at -20°C did not significantly change the receptor binding. Sonication thus appears to be a useful first step in purifying the opiate receptor.

AB - Sonication of rat brain membranes for 9 minutes solubilized 35% of their stereospecific opiate binding activity; a second 9 minute sonication of the insoluble residue released an additional 21% of the original binding. The opiate binding properties of the solubilized material were highly similar to those of membrane bound receptor by a number of criteria, including affinity, effect of sodium, and the IC50 of unlabeled opiates in displacing 3H-etorphine binding. Moreover, storage of the solubilized receptor fraction for two weeks at -20°C did not significantly change the receptor binding. Sonication thus appears to be a useful first step in purifying the opiate receptor.

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Solubilization of membrane bound opiate receptor from rat brain

Abstract

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